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Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein

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Biologia Plantarum

Abstract

Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm−3 hygromycin. Expression of green fluorescent protein (GFP) was observed in the callus and protocorm-like body (PLBs) tissues survived on the selection medium. The presence of green fluorescence protein (sgfp), hygromycin-B-phosphotransferase (hptII) and β-glucuronidase (uidA) genes in the transformed tissues were verified using PCR, Southern blot and dot blot analyses. Based on the results from PCR and expression of sgfp and uidA genes in the calluses and PLBs survived from hygromycin selection, we reported the co-transformation of sgfp, hptII and uidA genes into Dendrobium Sonia 17. GFP-expressing tissues were also observed in the regenerated transformed plantlets.

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Abbreviations

GFP:

green fluorescent protein

GUS:

β-glucuronidase

MS:

Murashige and Skoog

PLBs:

protocorm-like bodies

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Acknowledgements

The authors wish to thank Dr. J. Sheen (Boston, USA) for kindly providing the GFP constructs and Dr. S. Manickam (Universiti Putra Malaysia) for the pSMDFR plasmid. This research was supported by Malaysia government’s IRPA MOSTE- TOP-DOWN project # 01-02-04-T001.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    C. S. Tee, M. Maziah & M. P. Abdullah

  2. Malaysian Agricultural Research and Development Institute, 43400, Serdang, Selangor, Malaysia

    C. S. Tan

  3. Department of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900, Kampar, Perak, Malaysia

    C. S. Tee

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  1. C. S. Tee
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  2. M. Maziah
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  3. C. S. Tan
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  4. M. P. Abdullah
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Correspondence to C. S. Tee.

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Tee, C.S., Maziah, M., Tan, C.S. et al. Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein. Biol Plant 55, 572–576 (2011). https://doi.org/10.1007/s10535-011-0128-1

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  • DOI: https://doi.org/10.1007/s10535-011-0128-1

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