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Changes of leaf antioxidant system, photosynthesis and ultrastructure in tea plant under the stress of fluorine

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Biologia Plantarum

Abstract

Seedlings of Camellia sinensis were grown hydroponically for 30 d in order to study the effect of fluorine (F) on growth parameters, antioxidant defence system, photosynthesis and leaf ultrastructure. Fresh and dry mass, chlorophyll (Chl) content and net photosynthetic rate (PN) decreased with increasing F concentration. Superoxide dismutase (SOD) activity decreased significantly, catalase (CAT) and guaiacol peroxidase (GPX) activities reached maximun under 0.21 and 0.32 mM F, respectively. Proline, malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents increased significantly. These results suggested, that antioxidant defence system of leaves did not sufficiently scavenge excessive reactive oxygen species. The cell ultrastructure was not changed under 0.11–0.21 mM F, however, it was destroyed at 0.32–0.53 mM F. So tea plants tolerated F in concentration less than 0.32 mM.

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Abbreviations

CAT:

catalase

Chl:

chlorophyll

F:

fluorine

GPX:

guaiacol peroxidase

MDA:

malondialdehyde

PN :

net photosynthetic rate

SOD:

superoxide dismutase

TEM:

transmission electron microscope

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Acknowledgements

Authors are grateful to Prof. S.Y. Lan for his helpful suggestions and guidance in transmission electron microscope.

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Authors and Affiliations

  1. College of Horticulture and Forestry Science, Huazhong Agricultural University, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Shizishan Street, Wuhan, 430070, P.R. China

    C. Li, Y. Zheng, J. Zhou, J. Xu & D. Ni

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  1. C. Li
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  2. Y. Zheng
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  3. J. Zhou
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  4. J. Xu
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  5. D. Ni
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Correspondence to D. Ni.

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Li, C., Zheng, Y., Zhou, J. et al. Changes of leaf antioxidant system, photosynthesis and ultrastructure in tea plant under the stress of fluorine. Biol Plant 55, 563–566 (2011). https://doi.org/10.1007/s10535-011-0126-3

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  • DOI: https://doi.org/10.1007/s10535-011-0126-3

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