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cDNA-AFLP profiling in the embryo axes during common bean germination

Biologia Plantarum

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Abstract

cDNA-AFLP fingerprinting was used to identify genes with modulated expression during germination in common bean (Phaseolus vulgaris L.). The analysis was performed on the embryo axes. Nine time points covering the whole germination were considered and 800 transcript-derived fragments (PvTDFs) were scored. Among them, 80 % showed no changes during germination. The 97 PvTDFs showing differential expressions during germination were sequenced along with 14 constant transcripts that were randomly chosen. The expression of seven variable PvTDFs was confirmed by real-time RT-PCR. We observed that 92 % of the transcript changes, including 35 % of appearing mRNAs, took place before radicule protrusion, 0–17 h after imbibition (HAI). A major shift in gene expression was observed between 9 and 14 HAI, suggesting a key moment of cell re-programming. Sequence homologies were found for 52 % of the sequenced PvTDFs. The identified transcripts encode proteins belonging to several functional groups including transcription factors, proteins involved in storage compound hydrolysis, cell elongation or oxidative stress protection.

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Abbreviations

BLAST :

basic local alignment search tool

cDNA-AFLP:

cDNA-amplified fragment length polymorphism

Ct:

cycle threshold

HAI:

hour after imbibition

PCR:

polymerase chain reaction

PvTDF:

Phaseolus vulgaris transcript-derived fragment

RE:

relative expression

RT-PCR:

reverse transcription-PCR

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Acknowledgements

This work was supported by a grant from the Contrat de Plan Etat-Région des Pays-de-la Loire 2000–2006. We thank Dr. M. Hundertmark (Laboratory of Molecular Seed Physiology in Angers) for reading this manuscript.

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  1. D. Peltier

    Present address: IFR 149 Quasav, UMR 1259 GENHORT, 2, bd. Lavoisier, F-49045, Angers Cedex 01, France

Authors and Affiliations

  1. UMR 1191 Physiologie Moléculaire des Semences, Agrocampus Oues, IFR 149 Quasav, 16, bd. Lavoisier, F-49045, Angers Cedex 01, France

    B. Teulat-Merah

  2. UMR 1191 Physiologie Moléculaire des Semences, Université d’Angers, IFR 149 Quasav, 16, bd. Lavoisier, F-49045, Angers Cedex 01, France

    M. -C. Morere-Le Paven, C. Ricoult, C. Aubry & D. Peltier

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  1. B. Teulat-Merah
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  3. C. Ricoult
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Teulat-Merah, B., Morere-Le Paven, M.C., Ricoult, C. et al. cDNA-AFLP profiling in the embryo axes during common bean germination. Biol Plant 55, 437–447 (2011). https://doi.org/10.1007/s10535-011-0108-5

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