Isolation and characterization of eleven polymorphic microsatellite loci in Aegiphila sellowiana and their transferability
We isolated and characterized eleven polymorphic microsatellite loci for Aegiphila sellowiana an outcrossing pioneer tree species that is frequently used in reforestation programs of tropical riparian forests in Brazil. A total of 38 alleles were detected across a sample of 45 individuals of A. sellowiana, with an average number of 3.45 alleles per locus. The average polymorphic information content (PIC) was 0.430 and the observed (HO) and expected (HE) heterozygosity values varied from 0.156 to 1.000 and 0.145 to 0.730, respectively. Eight loci exhibited significant deviation from Hardy-Weinberg equilibrium (P ≤ 0.001) and 32 pair combinations of loci showed significant linkage disequilibrium (P ≤ 0.001). All 11 primers were tested for cross amplification in 12 species belonging to the family Lamiaceae and 5 species belonging to the related family Verbenaceae. The sequence and diversity information obtained using these microsatellites and their cross-transferability to other species of Lamiaceae as well as Verbenaceae will increase our understanding of genetic structures and species relationships within Aegiphyla and other genera of these families.
Additional key wordscross amplification genetic diversity Lamiaceae microsatellite primers Verbenaceae
microsatellite or simple sequence repeat
polymerase chain reaction
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This work is supported by a grant from the Conselho Nacional de Pesquisa (CNPq, process number 478179/2006-5), Fundação Araucária (Process number 064/2007) and the Coordenação de Aperfeiçoamento de pessoal de Nível Superior (CAPES) for the PhD grants to Eduardo A. Ruas.
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