Abstract
The blue colour of Vanda coerulea petals is slowly produced during flower development but quickly disappears when the flowers are pollinated. To investigate the molecular basis of the phenomenon, we isolated a novel cytochrome P450 gene, CYP76AB1, from this plant by polymerase chain reaction (PCR) based on the conserved regions of flavonoid 3′,5′-hydroxylase amino acid sequences. CYP76AB1 transcripts were detectable at low level in the late phase of flower development when the petals were light blue, but became abundant in the subsequent opening flower stage, as the petals turned blue. When the flowers were pollinated they turned white, and the CYP76AB1 transcripts returned to low level. This indicates that the expression of CYP76AB1 may regulate petal colour of V. coerulea, and be a target for developing permanently blue flowers.
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Abbreviations
- cDNA:
-
complementary deoxyribonucleic acid
- CYP75A:
-
cytochrome P450, family 75, subfamily A
- CYP76AB1:
-
cytochrome P450, family 76, subfamily AB, isoform 1
- CYP93B:
-
cytochrome P450, family 93, subfamily B
- dNTP:
-
deoxynucleotide triphosphate
- F3′H:
-
flavonoid 3′-hydroxylase
- F3′5′H:
-
flavonoid 3′,5′-hydroxylase
- FNSII:
-
flavone synthase II
- gDNA:
-
genomic deoxyribonucleic acid
- P450s:
-
cytochromes P450
- ORF:
-
open reading frame
- RACE:
-
rapid amplification of cDNA end
- RT-PCR:
-
reverse transcriptase polymerase chain reaction
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Acknowledgements
This work was supported by Coordinating Center for Thai Government Science and Technology Scholarship Students, National Science and Technology Development Agency, Ministry of Science and Technology, Thailand (F-31-312-79-01).
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Ratanasut, K., Wongkhamprai, B. & Maknoi, S. Expression of a CYP76AB1 correlates with the sequential white-blue-white colour transition of Vanda coerulea petals. Biol Plant 55, 353–356 (2011). https://doi.org/10.1007/s10535-011-0053-3
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DOI: https://doi.org/10.1007/s10535-011-0053-3