Genetic variation within and among small isolated populations of Santalum album
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A combination of directed amplification of minisatellite DNA (DAMD) and random amplification of polymorphic DNA (RAPD) primes were used to assess the genetic variation within and between three isolated populations of Indian sandalwood (Santalum album). Eleven primers used in this study amplified 65.99 % polymorphic bands. Analysis of molecular variance revealed a high genetic variation among these populations (ϕST = 0.549). There are indications of clonality within the existing Indian sandalwood populations which can be attributed to habitat fragmentation, isolation and vegetative reproduction.
Additional key wordsDAMD genetic diversity population fragmentation RAPD sandalwood
analysis of molecular variance
directed amplification of minisatellite DNA
random amplification of polymorphic DNA
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We thank Dr. S.R. Bhat, NRCPB, New Delhi for his valuable comments on the manuscript. Authors thank Mr. K.S. Sanjith and Mr. Krishnappa for their help in field work. Financial assistance by Department of Biotechnology, Government of India (Grant number BT/PR6739/PBD/17/433/2005) is duly acknowledged.
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