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In vitro regeneration of Solanum nigrum with enhanced solasodine production

Abstract

High frequency of shoot formation was achieved from Solanum nigrum L. leaves on Murashige and Skoog (MS) medium without any callusing stage. Shoot forming ability was more pronounced on leaves positioned dorsally. For shoot induction, 2.0 mg dm−3 benzylaminopurine and 1.5 mg dm−3 kinetin were observed to be the most effective plant growth regulators (PGRs). The present paper also describes first successful induction of in vitro flowering in S. nigrum. The leaf derived shoots were excised and treated with various root promoting PGRs and 0.25 mg dm−3 indole-3-butyric acid produced maximum number of roots (15.2 per plant). Plants were later transplanted in field with 100 % survival. Solasodine content was higher in in vitro raised shoots and leaf derived callus, compared to ex vitro grown shoots.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog

NAA:

α-naphthaleneacetic acid

PGRs:

plant growth regulators

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Acknowledgements

The first author is highly thankful to Hamdard National Foundation for receiving financial assistance during the experimental work. Our laboratory colleagues and technical staffs of the Department are also acknowledged for their support and help.

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Correspondence to A. Mujib.

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Bhat, M.A., Mujib, A., Junaid, A. et al. In vitro regeneration of Solanum nigrum with enhanced solasodine production. Biol Plant 54, 757–760 (2010). https://doi.org/10.1007/s10535-010-0136-6

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Additional key words

  • black nightshade
  • HPTLC
  • in vitro flowering
  • plant growth regulators