Abstract
Effect of gibberellic acid (GA3) combined with saponin on shoot elongation of Asparagus officinalis was evaluated in tissue culture. Addition of saponin to GA3 supplemented Murashige and Skoog (MS) basal medium showed a dose depended effect on shoot length of Asparagus officinalis. However, increasing concentration of saponin above 3.0 mg dm−3 decreased shoot length and showed yellowing and thinning of shoots. Saponin (3.0 mg dm−3) + GA3 (0.2 mg dm−3) mixture treated by variable duration of sonication (0, 1, 3, 5, 7, 9, 11, 13 and 15 min) were evaluated for shoot elongation on MS basal medium. The highest shoot lengths (14.4 ± 0.3 and 15.1 ± 0.1 cm) were found after 5 and 7 min sonication.
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BAP:
-
6-benzylaminopurine
- GA3 :
-
gibberellic acid
- IBA:
-
indole 3-butyric acid
- Kin:
-
kinetin
- MS:
-
Murashige and Skoog
References
Armah, C.N., Mackie, A.R., Roy, C., Price, K., Osbourn, A.E., Bowyer, P., Ladha, S.: The membrane permeabilising effect of avenacin A-1 involves the reorganisation of bilayer cholesterol. — Biophys. J. 76: 281–290, 1999.
Bishnu, B.P., Wiesman, Z.: Phyto-saponins as a natural adjuvant for delivery of agromaterials through plant cuticle membranes. — J. Agr. Food Chem. 54: 6277–6285, 2006.
Gregoriadis, G., McCormack, B., Morrison-Perrie, Y., Saffie, R., Zadi, B.: Liposome in drug targeting. — In: Celis, J. (ed.): Cell Biology: Laboratory Handbook. Pp. 131–140. Academic Press, New York 1998.
Keukens, E.A.J., Vrje, T., Van den Boom, C., Demel, R.A., Jongen, W.M.F., Kruijff, B.: Molecular basis of glycoalkaloid induced membrane disruption. — Biochim. biophys. Acta 1240: 216–228, 1995.
Morrissey, J.P., Osbourn, A.E.: Fungal resistance to plant antibiotics as a mechanism of pathogenesis. — Microbiol. mol. Biol. Rev. 63: 708–724, 1999.
Murashige, T., Skoog, F.: A revised medium of rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.
Nishikawa, M., Nojima, S., Akiyama, T., Sankawa, U., Inoue, K.: Interaction of digitonin and its analogs with membrane cholesterol. — J. Biochem. 96: 1231–1239, 1984.
Oldenburg, K., Pooler, D., Scudder, K., Lipinski, C., Kelly, M.: High throughput sonication: evaluation for compound solubilization. — Combinatorial Chem. High Throughput Screen. 8: 499–512, 2005.
Oleszek, W.A., Hoagland, R.E., Zablotowicz. R.M.: Ecological significance of plant saponins. — In: Inderjit, Dakshini, K.M.M., Foy, C.L. (ed.): Principles and Practices of Plant Ecology. Allelochemical interactions. Pp. 451–465. CRC Press, London 1999.
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Saharan, V. Effect of gibberellic acid combined with saponin on shoot elongation of Asparagus officinalis . Biol Plant 54, 740–742 (2010). https://doi.org/10.1007/s10535-010-0132-x
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DOI: https://doi.org/10.1007/s10535-010-0132-x