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Induction of tetraploidy in Juncus effusus by colchicine

  • Original Papers
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Biologia Plantarum

Abstract

Tetraploidy was induced in vitro in mat rush (Juncus effusus L.) cultivar Nonglin-4 by exposure to colchicine (0, 50, 100 and 500 mg dm−3) for 6, 12 and 24 h. Flow cytometric analysis was used to confirm the ploidy level. Anatomical and ultrastructural analyses at cellular and subcellular levels in tetraploid and diploid control plants revealed differences between diploid and tetraploid plants. The leaf epidermis had larger stomata but lower stomatal density in tetraploid plants. In addition, mesophyll cells in tetraploid plants appeared more compact and showed less intercellular spaces along with increased size of vascular bundles. However, a significant reduction of chlorophyll content was observed in tetraploid plants that might be the result of structural modification in the lamellar membranes of chloroplasts.

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Abbreviations

MS:

Murashige and Skoog

BA:

benzylaminopurine

FCM:

flow cytometry

Cu:

cuticle

Epi:

epidermis

Mes:

mesophyll

Vas:

vascular bundle

CH:

chloroplast

CW:

cell wall

NU:

nucleus

VAC:

vacuole

PG:

plastoglobuli

TH:

thylakoid membrane

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Acknowledgements

This work was supported by the National High Technology Research and Development Program of China (2006AA10A214, 2006AA10Z234), the National Natural Science Foundation of China (30871652), Zhejiang Provincial Natural Science Foundation (R307095, Y306034), and the Postdoctoral Research Fund of Zhejiang University.

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Authors and Affiliations

  1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China

    L. Xu, U. Najeeb, M. S. Naeem, M. K. Daud, J. S. Cao, H. J. Gong & W. J. Zhou

  2. The University of Nottingham Ningbo, Ningbo, 315100, China

    W. Q. Shen

Authors
  1. L. Xu
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  2. U. Najeeb
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  3. M. S. Naeem
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  4. M. K. Daud
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  5. J. S. Cao
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  6. H. J. Gong
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  7. W. Q. Shen
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  8. W. J. Zhou
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Correspondence to W. J. Zhou.

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Xu, L., Najeeb, U., Naeem, M.S. et al. Induction of tetraploidy in Juncus effusus by colchicine. Biol Plant 54, 659–663 (2010). https://doi.org/10.1007/s10535-010-0117-9

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  • DOI: https://doi.org/10.1007/s10535-010-0117-9

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