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Activity of antioxidant enzyme during in vitro organogenesis in Crocus sativus

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Biologia Plantarum

Abstract

The effect of various hormonal combinations on regeneration of shoots and roots from meristem-derived callus of Crocus sativus L. and activities of antioxidant enzymes have been studied. The most efficient regeneration occurred with 1.0 mg dm−3 1-naphthaleneacetic acid (NAA) + 1.0 mg dm−3 thidiazuron and 1.0 mg dm−3 NAA + 2.0 mg dm−3 kinetin. For sprouting, regenerated shoot were subcultured on Murashige and Skoog medium containing 1.0 mg dm−3 NAA + 1.0 mg dm−3 benzylaminopurine (BAP). Protein content and superoxide dismutase activity decreased in regenerated shoots and roots and increased in sprouting shoots, while catalase (CAT), peroxidase (POX) and polyphenol oxidase (PPO) activities increased during organogenesis and decreased in sprouting shoots. High CAT and PPO activities were detected in regenerated roots, whereas high POX activity was observed in regenerated shoot.

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Abbreviations

BAP:

N6-benzylaminopurine

CAT:

catalase

EDTA:

ethylenediaminetetraacetic acid

KIN:

kinetin

L-DOPA:

3,4-dihydroxy-L-phenylalanin

MS:

Murashige and Skoog

NAA:

1-naphthaleneacetic acid

NBT:

nitroblue tetrazolium

PAGE:

polyacrylamide gel electrophoresis

PIC:

picloram (4-amino-3,5-trichloropicolinic acid)

POX:

peroxidase

PPO:

polyphenol oxidase

PVPP:

polyvinylpolypyrrolidone

ROS:

reactive oxygen species

SDS:

sodium dodecyl sulfate

SOD:

superoxide dismutase

TDZ:

thidiazuron

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Acknowledgements

The financial support of this research was provided by University of Tehran. The authors are grateful to the anonymous reviewers for very helpful and valuable advices.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Biology, College of Sciences, University of Tehran, Tehran, 14155, I.R. Iran

    E. Vatankhah, V. Niknam & H. Ebrahimzadeh

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  1. E. Vatankhah
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  2. V. Niknam
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  3. H. Ebrahimzadeh
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Correspondence to V. Niknam.

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Vatankhah, E., Niknam, V. & Ebrahimzadeh, H. Activity of antioxidant enzyme during in vitro organogenesis in Crocus sativus . Biol Plant 54, 509–514 (2010). https://doi.org/10.1007/s10535-010-0089-9

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  • DOI: https://doi.org/10.1007/s10535-010-0089-9

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