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Biologia Plantarum

, Volume 54, Issue 3, pp 483–487 | Cite as

Contents of polyamines during vernalization in wheat and the effect of zearalenone

  • M. Filek
  • J. Biesaga-Kościelniak
  • I. Marcińska
  • M. Cvikrová
  • I. Macháčková
  • J. KrekuleEmail author
Original Papers

Abstract

The contents of endogenous free and conjugated polyamines, putrescine (Put) and spermidine (Spd), were determined during 9 week of vernalization (at 5 °C) in winter wheat seedlings cultivated on Murashige and Skoog media without (MS0) and with 2 mg dm−3 zearalenone (MSZEN). At the 4th week of chilling treatment, which is sufficient to induce generative development in 30 % of plants, the marked increase in free and conjugated forms of Put and free Spd were observed. The presence of ZEN in medium significantly accelerated the vernalization. About 20 % of plants treated with ZEN flowered already after 2 weeks and 40 % after 3 weeks of chilling. Significantly higher content of free Put was determined in roots grown on MSZEN compared with MS0 during the first 5 weeks of vernalization with maximum at the 4th week. After germination, a marked decrease in free Spd content was observed both in plants grown on MS0 and MSZEN. Application of ZEN significantly slowed down the Spd decline in leaves and roots during the first and second week of vernalization. The content of Spd and its conjugates decreased in vernalized plants after 1 week of cultivation at 20 °C.

Additional key words

flowering polyamine conjugates putrescine spermidine Triticum aestivum 

Abbreviations

MS

Murashige and Skoog

ZEN

zearalenone

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Notes

Acknowledgements

This work was supported by Institutional Grant AV0Z 50380511 and by Ministry of Education, the project OC 08013, both of Czech Republic.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • M. Filek
    • 1
    • 2
  • J. Biesaga-Kościelniak
    • 1
  • I. Marcińska
    • 1
  • M. Cvikrová
    • 3
  • I. Macháčková
    • 3
  • J. Krekule
    • 3
    Email author
  1. 1.Institute of Plant PhysiologyPolish Academy of SciencesKrakówPoland
  2. 2.Institute of BiologyPedagogical UniversityKrakówPoland
  3. 3.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicPragueCzech Republic

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