Contents of polyamines during vernalization in wheat and the effect of zearalenone
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The contents of endogenous free and conjugated polyamines, putrescine (Put) and spermidine (Spd), were determined during 9 week of vernalization (at 5 °C) in winter wheat seedlings cultivated on Murashige and Skoog media without (MS0) and with 2 mg dm−3 zearalenone (MSZEN). At the 4th week of chilling treatment, which is sufficient to induce generative development in 30 % of plants, the marked increase in free and conjugated forms of Put and free Spd were observed. The presence of ZEN in medium significantly accelerated the vernalization. About 20 % of plants treated with ZEN flowered already after 2 weeks and 40 % after 3 weeks of chilling. Significantly higher content of free Put was determined in roots grown on MSZEN compared with MS0 during the first 5 weeks of vernalization with maximum at the 4th week. After germination, a marked decrease in free Spd content was observed both in plants grown on MS0 and MSZEN. Application of ZEN significantly slowed down the Spd decline in leaves and roots during the first and second week of vernalization. The content of Spd and its conjugates decreased in vernalized plants after 1 week of cultivation at 20 °C.
Additional key wordsflowering polyamine conjugates putrescine spermidine Triticum aestivum
Murashige and Skoog
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This work was supported by Institutional Grant AV0Z 50380511 and by Ministry of Education, the project OC 08013, both of Czech Republic.
- Biesaga-Kościelniak, J.: Zearalenone as a New Hypothetical Regulator of Plant Growth and Development. — Inst. Plant Physiol., Polish Acad. Sci., Krakow 2001.Google Scholar
- Caffaro, S.V., Vicente, C.: Early changes in the content of leaf polyamines during the photoperiodic flowering induction in soybean. — J. Plant Physiol. 145: 756–758, 1995.Google Scholar
- Filek, M., Biesaga-Kościelniak, J., Macháčková, I., Krekule, J.: Generative development of winter rape (Brassica napus L.) — the role of vernalization. — Int. J. Plant dev. Biol. 1: 57–63, 2007.Google Scholar
- Fu, Y.F., Li, H.Y., Meng, F.J.: The possible role of zearalenone in the floral gradient in Nicotiana tabacum L. — J. Plant Physiol. 147: 197–202, 1995.Google Scholar
- Liu, J.H., Kitashiba, H., Wang, J., Ban, Y., Moriguchi, T.: Polyamines and their ability to provide environmental stress tolerance to plants. — Plant Biotechnol. 24: 117–126, 2007.Google Scholar
- Meng, F.J., Han, Y.Z., Fu, Y.F., Guo, F.L.: Zearalenone in higher plants. — Flowering Newslett. 22: 54–57, 1996.Google Scholar
- Meng, F.J., Han, Y.Z., Que, Y.M., Wang, H.: Zearalenone, a key substance controlling plant development. — In: Karssen, C.M., Van Loon, L.C., Vreuggdeunilcedes, D. (ed.): Advances in Plant Regulation. Pp 291–297. Kluwer Academic Press, Dordrecht 1992.Google Scholar
- Meng, F.J., Que, Y.M., Zhang, S.Q.: Zearalenone-like substance in winter plants and its relation to vernalization. — Acta bot. sin. 28: 622–627, 1986.Google Scholar
- Páldi, E., Janda, T., Szalai, G: Quantitative changes in polyamines during vernalization in wheat (Triticum aestivum L.). — Növénytermelés 47: 613–622, 1998.Google Scholar
- Rácz, I., Kovács, M., Lasztity, D., Veisz, O., Szalai G., Páldi, E.: Effect of short-term and long-term low temperature stress on polyamine biosynthesis in wheat genotypes with varying degrees of frost tolerance. — Plant Physiol. 148: 368–373, 1996.Google Scholar