Abstract
The analyses of some antioxidant enzyme activities were carried out in the course of strawberry fruits development and ripening. The catalase activity was maximum in small-sized green fruits, it decreased in middle-sized green fruits and increased again during the ripening stages. The highest superoxide dismutase and peroxidase activities were observed in white fruits.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- CAT:
-
catalase
- NBT:
-
nitroblue tetrazolium
- OPD:
-
o-phenylenediamine
- POX:
-
peroxidase
- ROS:
-
reactive oxygen species
- SOD:
-
superoxide dismutase
References
Aebi, H.: Catalase. — Methods Enzymol. 3: 273–282, 1983.
Aharoni, A., Keizer, L.C.P., Van den Broeck, H.C., Blanco-Portales, R., Munoz-Blanco, J., Bois, G., Smit, P., De Vos, R., O’Connell, A.P.: Novel insight into vascular, stress, and auxin-dependent and -independent gene expression programs in strawberry, a non climacteric fruit. — Plant Physiol. 129: 1019–1031, 2002.
Aharoni, A., O’Connell, A.P.: Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays. — J. exp. Bot. 53: 2073–2087, 2002.
Anand, T., Ghaskaran, R., Raguchander, T., Samiyappan, R., Prakasan, V., Gopalakrishnan, C.: Defence responses of chilli fruits to Colletotrichum capsici and Alternaria alternata. — Biol. Plant. 53: 553–559, 2009.
Beauchamp, C., Fridovich, I.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gel. — Anal. Biochem. 44: 276–287, 1971.
Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. — Anal. Biochem. 72: 248–254, 1976.
Civello, P.M., Martínez, G.A., Chaves, A.R., Anon, M.C.: Peroxidase from strawberry fruit (Fragaria × ananassa Duch.): partial purification and determination of some properties. — J. Agr. Food Chem. 43: 2596–2601, 1995.
Foyer, C.H., Descourvieres, P., Kunert, K.J.: Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. — Plant Cell Environ. 17: 507–523, 1994.
Given, N.K., Venis, M.A., Grierson, D.: Hormonal regulation of ripening in the strawberry, a non climacteric fruit. — Planta 174: 402–406, 1998.
Hernandez, J.A., Jimenez, A., Mullineaux, P., Sevilla, F.: Tolerance of pea (Pisum sativum) to long term salt stress is associated with induction of antioxidant defences. — Plant Cell Environ. 23: 853–862, 2000.
Jimenez, A., Romojaro, F., Gomez, J.M., Llanos, M.R., Sevilla, F.: Antioxidant systems and their relationship with the response of pepper fruits to storage at 20 °C. — J. Agr. Food Chem. 51: 6293–6299, 2003.
Leprince, O., Hoekstra, F.A., Harren, F.J.M.: Unravelling the responses of metabolism to dehydration points to a role for cytoplasmic viscosity in desiccation tolerance. — In: Black, M, Bradford, K.J., Vasques-Ramos, J. (ed.): Seed Biology: Advances and Applications. Pp. 57–66. CABI Publishing, Wallingford 2000.
Lopez-Serrano, M., Barcelo, A.R.: Histochemical localization and developmental expression of peroxidase and polyphenol oxidase in strawberries. — J. amer. Soc. hort. Sci. 126: 27–32, 2001.
Manning, K.: Changes in gene expression during strawberry fruit ripening and their regulation by auxin. — Planta 194: 62–68, 1994.
Manning, K.: Isolation of a set of ripening-related genes from strawberry: their identification and possible relationship to fruit quality traits. — Planta 205: 622–635, 1998.
Nitsch, J.P.: Growth and morphogenesis of the strawberry as related to auxin. — Amer. J. Bot. 37: 211–215, 1950.
Normanly, J., Slovin, J.P., Cohen, J.D.: Rethinking auxin biosynthesis and metabolism. — Plant Physiol. 107: 323–329, 1995.
Olsson, M.E., Ekvall, J., Gustavsson, K.E., Nilsson, J., Pillai, D., Sjöholm, I., Svensson, U., Akesson, B., Nyman, M.G.L.: Antioxidant, low molecular weight carbohydrates and total antioxidant capacity in strawberries (Fragaria × ananassa): effects of cultivar, ripening and storage. — J. Agr. Food Chem. 52: 2490–2498, 2004.
Önnerud, H., Zhang, L., Gellerstedt, G., Henriksson, G.: Polymerization of monolignols by redox shuttle-mediated enzymatic oxidation: a new model in lignin biosynthesis I. — Plant Cell 14: 1953–1962, 2002.
Pastori, G.M., Del Rio, L.A.: Natural senescence of pea leaves: an activated oxygen-mediated function for peroxisomes. — Plant Physiol. 113: 411–418, 1997.
Procházková, D., Wilhelmová, N.: Leaf senescence and activities of the antioxidant enzymes. — Biol. Plant. 51: 401–406, 2007.
Rogiers, S.Y., Kumar, M.G.N., Knowles, N.R.: Maturation and ripening of fruit Amelanchier alnifolia Nutt. are accompanied by increasing oxidative stress. — Ann. Bot. 81: 203–211, 1998.
Sairam, R.K., Saxena, D.C.: Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. — J. Agron. Crop Sci. 184: 55–61, 2000.
Sairam, R.K., Srivastava, G.C.: Water stress tolerance of wheat (Triticum aestivum L.): variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes. — J. Agron. Crop Sci. 186: 63–70, 2001.
Sairam, R.K., Srivastava, G.C., Saxena, D.C.: Increased antioxidant activity under elevated temperature: a mechanism of heat stress tolerance in wheat genotypes. — Biol. Plant. 43: 245–251, 2000.
Seymour, G.B., Taylor, J.E., Tucker, G.A.: Biochemistry of Fruit Ripening. — Chapman and Hall, London 1993.
Thompson, J.E., Ledge, R.L., Barber, R.F.: The role of free radicals in senescence and wounding. — New Phytol. 105: 317–344, 1987.
Acknowledgements
The authors thanks to Dr. Agustin González-Fontes for valuable comments on the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
López, A.P., Gochicoa, M.T.N. & Franco, A.R. Activities of antioxidant enzymes during strawberry fruit development and ripening. Biol Plant 54, 349–352 (2010). https://doi.org/10.1007/s10535-010-0061-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10535-010-0061-8