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Somatic embryogenesis from rhizome explants of Cymbopogon winterianus

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Biologia Plantarum

Abstract

Plantlet regeneration through indirect somatic embryogenesis was attempted from rhizome derived callus of Cymbopogon winterianus Jowitt (cv. Jorlab2). Optimum callus was induced on Murashige and Skoog (MS) basal medium supplemented with 4 mg dm−3 2,4-dichlorophenoxyacetic acid (2,4-D). Initially the callus was friable, shiny white and watery in nature. After subculturing on MS medium containing 2,4-D and kinetin (Kn), callus was transferred onto the MS medium supplemented with 2,4 -D, Kn and coconut water to induce somatic embryogenesis. Optimum somatic embryogenesis (78.33 %) was achieved on MS medium containing 3.0 mg dm−3 2,4-D and 0.5 mg dm−3 Kn. High frequency (65 %) plantlet conversion from embryos was achieved in MS medium supplemented with 2 mg dm−3 N6-benzyladenine (BA), 0.5 mg dm−3 Kn, 0.2 mg dm−3 calcium pantothenate and 0.2 mg dm−3 biotin.

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Abbreviations

BA:

benzyladenine

CaP:

calcium pantothenate

2,4-D:

2,4-dichlorophenoxyacetic acid

Kn:

kinetin

MS:

Murashige and Skoog

RM:

regeneration medium

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Acknowledgments

We wish to thank Dr. T. K. Bandopadhyay, Head, Department of Molecular Biology and Biotechnology, University of Kalyani for valuable suggestions and encouragements. This study was supported by UGC, New Delhi, India.

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Authors and Affiliations

  1. Cytogenetics and Plant Biotechnology Research Unit, Department of Botany, University of Kalyani, Kalyani, 741235, India

    T. Dey, S. Bhattacharya & P. D. Ghosh

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  1. T. Dey
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  2. S. Bhattacharya
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  3. P. D. Ghosh
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Correspondence to P. D. Ghosh.

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Dey, T., Bhattacharya, S. & Ghosh, P.D. Somatic embryogenesis from rhizome explants of Cymbopogon winterianus . Biol Plant 54, 325–328 (2010). https://doi.org/10.1007/s10535-010-0056-5

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  • DOI: https://doi.org/10.1007/s10535-010-0056-5

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