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The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron

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Biologia Plantarum

Abstract

The influence of cytokinin thidiazuron (TDZ) and auxin indole-3-acetic acid (IAA) on in vitro shoot organogenesis of fifteen Rhododendron genotypes was investigated and a protocol for high frequency adventitious shoot regeneration from leaf explants was developed. High genotypic variation was observed and regeneration frequencies ranged from 0 to 100 %. Genotype Ovation had the highest number of shoots (26.4 per explant) after 12 weeks on medium with 0.57 µM IAA and 1.20 µM TDZ, but only 65 % of explants regenerated. Catawbiense Grandiflorum had 17.7 shoots per explant and 75 % regeneration on medium with 5.70 µM IAA and 0.45 µM TDZ and Van Werden Poelman had 14.3 shoots per explant and 100 % regeneration on medium with 0 57 µM IAA and 0.45 µM TDZ.

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Abbreviations

IAA:

indole-3-acetic acid

PVP:

polyvinylpyrrolidone

TDZ:

thidiazuron

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Authors and Affiliations

  1. Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, CZ-37005, České Budějovice, Czech Republic

    D. Pavingerová

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  1. D. Pavingerová
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Correspondence to D. Pavingerová.

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Acknowledgements:This work was supported by AV0Z50510513.

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Pavingerová, D. The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron . Biol Plant 53, 797–799 (2009). https://doi.org/10.1007/s10535-009-0147-3

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  • DOI: https://doi.org/10.1007/s10535-009-0147-3

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