Abstract
The development of chloroplast microsatellite (cpSSR) markers in Cucumis species and analysis of their polymorphism and transferability were reported. Fifteen microsatellite markers, represented by mononucleotide repeats, were developed from the complete sequence of Cucumis sativus chloroplast genome. Intraspecific variation was successfully detected in C. sativus and C. melo and revealed mean 1.6 and 1.9 alleles per cpSSR locus, respectively. With the exception of two exon region-located cpSSR markers being monomorphic, each of the others amplified polymorphic fragments in C. sativus or C. melo. A total of 34 polymorphic loci were detected with these cpSSR markers in the two species. Transferability of the newly developed cpSSR markers was checked on an additional set of 41 Cucurbitaceae accessions (belonging to 12 different species), and except for two markers with no amplification in Cucurbita maxima, the others could be transferable to all the accessions tested. Of the 15 cpSSR markers, 14 markers generated fragments with expected band sizes and 13 markers detected interspecific polymorphism among the accessions. Intraspecific polymorphism was also observed within four Cucurbitaceae species excluding C. sativus and C. melo.
Abbreviations
- SSR:
-
microsatellite or simple sequence repeat
- cpSSR:
-
chloroplast microsatellite
- RFLP:
-
restriction fragment length polymorphism
- CTAB:
-
cetyltrimethylammonium bromide
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Acknowledgements: This work is supported by a grant from the National High Technology Research and Development Program of China (863 Program, No. 2007AA10Z100). The authors are grateful to Prof. W.G. Liu and Dr. S.J. Zhao, Zhengzhou Fruit Institute, Chinese Academy of Agriculture Science, for their assistance in the laboratory work on this project.
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Hu, J.B., Zhou, X.Y. & Li, J.W. Development of novel chloroplast microsatellite markers for Cucumis from sequence database. Biol Plant 53, 793–796 (2009). https://doi.org/10.1007/s10535-009-0146-4
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DOI: https://doi.org/10.1007/s10535-009-0146-4