Abstract
To get deeper insight on the molecular mechanism underlying production of volatile compounds in apple (Malus domestica Borkh.), we performed the isolation and expression analysis of one R2R3-type MYB gene named MdMYBB. The amino acid sequence and the structural features of MdMYBB highly resembled those of PhODO1, which is a key regulator for floral scent biosynthesis in petunia. The expression of MdMYBB was repressed concomitantly with the inhibition of ethylene production, which regulates the volatile synthesis in apple. However, MdMYBB expression was not detected in the flesh from nearly ripened apple fruits, although the detection of exogenous volatiles had actually occurred in the same portion. In addition, overexpression of MdMYBB did not cause any induction of the volatile compounds in the transgenic tobacco lines. Thus, the features of MdMYBB were not in accordance with the aroma volatile emission, unlike the case of PhODO1, suggesting that MdMYBB may not be involved in the regulation of the biosynthesis for apple aroma volatiles. On the basis of the specific expression patterns, we discussed possible physiological roles of MdMYBB in apple.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- AAT:
-
alcohol acyltransferase
- AFS:
-
(E,E)-α-farnesene synthase
- DAFB:
-
days after full bloom
- DAT:
-
days after treatment
- DIG:
-
digoxigenin
- 1-MCP:
-
1-methylcyclopropene
- RACE:
-
rapid amplification of cDNA ends
- RT-PCR:
-
reverse transcription-PCR
References
Ban, Y., Honda, C., Hatsuyama, Y., Igarashi, M., Bessho, H., Moriguchi, T.: Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin. — Plant Cell Physiol. 48: 958–970, 2007.
Brackmann, A., Streif, J., Bangerth, F.: Relationship between a reduced aroma production and lipid-metabolism of apples after long-term controlled-atmosphere storage. — J. amer. Soc. hort. Sci. 118: 243–247, 1993.
Cunningham, D.G., Acree, T.E., Barnard, J., Butts, R., Braell, P.: Analysis of apple volatiles. — Food Chem. 19: 137–147, 1986.
Defilippi, B.G., Dandekar, A.M., Kader, A.A.: Relationship of ethylene biosynthesis to volatile production, related enzymes, and precursor availability in apple peel and flesh tissues. — J. Agr. Food Chem. 53: 3133–3141, 2005.
Girard, B., Lau, O.L.: Effect of maturity and storage on quality and volatile production of ‘Jonagold’ apples. — Food Res. Int. 28: 465–471, 1995.
Green, S., Friel, E.N., Matich, A., Beuning, L.L., Cooney, J.M., Rowan, D.D., MacRae, E.: Unusual features of a recombinant apple alpha-farnesene synthase. — Phytochemistry 68: 176–188, 2007.
Holland, D., Larkov, O., Bar-Ya’akov, I., Bar, E., Zax, A., Brandeis, E., Ravid, U., Lewinsohn, E.: Developmental and varietal differences in volatile ester formation and acetyl-CoA: alcohol acetyl transferase activities in apple (Malus domestica Borkh.) fruit. — J. agr. Food Chem. 53: 7198–7203, 2005.
Kobayashi, S., Ishimaru, M., Hiraoka, K., Honda, C.: Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis. — Planta 215: 924–933, 2002.
Li, D., Xu, Y., Xu, G., Gu, L., Li, D., Shu, H.: Molecular cloning and expression of a gene encoding alcohol acyltransferase (MdAAT2) from apple (cv. Golden Delicious). — Phytochemistry 67: 658–667, 2006.
Olias, J.M., Sanz, L.C., Rios, J.J., Perez, A.G.: Inhibitory effect of methyl jasmonate on the volatile ester-forming enzyme-system in Golden Delicious apples. — J. Agr. Food Chem. 40: 266–270, 1992.
Pechous, S.W., Whitaker, B.D.: Cloning and functional expression of an (E,E)-α-farnesene synthase cDNA from peel tissue of apple fruit. — Planta 219: 84–94, 2004.
Souleyre, E.J.F., Greenwood, D.R., Friel, E.N., Karunairetnam, S., Newcomb, R.D.: An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit flavor. — FEBS J. 272: 3132–3144, 2005.
Takos, A.M., Robinson, S.P., Walker, A.R.: Transcriptional regulation of the flavonoid pathway in the skin of dark-grown ‘Cripps’ Red’ apples in response to sunlight. — J. hort. Sci. Biotechnol. 81: 735–744, 2006.
Tatsuki, M., Endo, A.: Analyses of expression patterns of ethylene receptor genes in apple (Malus domestica Borkh.) fruits treated with or without 1-methylcyclopropene (1-MCP). — J. jap. Soc. hort. Sci. 75: 481–487, 2006.
Treutter, D.: Biosynthesis of phenolic compounds and its regulation in apple. — Plant Growth Regul. 34: 71–89, 2001.
Verdonk, J.C., Haring, M.A., Van Tunen, A.J., Schuurink, R.C.: ODORANT1 regulates fragrance biosynthesis in petunia flowers. — Plant Cell 17: 1612–1624, 2005.
Wan, C., Wilkins, T.A.: A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.). — Anal. Biochem. 223: 7–12, 1994.
Zimmermann, I.M., Heim, M.A., Weisshaar, B., Uhrig, J.F.: Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins. — Plant J. 40: 22–34, 2004.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ban, Y., Bessho, H. & Moriguchi, T. A putative PhODO1 homologous MYB transcription factor gene, MdMYBB, is not involved in the regulation of aroma volatile biosynthesis in apple. Biol Plant 53, 755–758 (2009). https://doi.org/10.1007/s10535-009-0138-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10535-009-0138-4