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AFLP analysis of somaclonal variations in Eucalyptus globulus

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Biologia Plantarum

Abstract

DNA variations of forty-eight Eucalyptus globulus plants, regenerated by successive culture from seven different explants were assessed by AFLP analysis using 18 primer combinations. At least one variation showed 66.7 % of the analyzed plants, and the numbers of polymorphic bands per plant ranged from 1 to 22. The proportion of polymorphic fragments did not correlate with the numbers of the regenerated plants. However, the more times of successive culture were done the more of polymorphic bands were found within the groups. On average, between 97.39 and 99.88 % of all fragments were shared within the same group. AMOVA analysis showed 39.33 % of the variation was found among the accessions that originated from different calli while 60.67 % was from same calli.

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Abbreviations

AFLP:

amplified fragment length polymorphism

ISSR:

inter-simple sequence repeat

RAPD:

random amplified DNA polymorphism

RFLP:

restriction fragment length polymorphism

UPGMA:

unweighted pair group method with arithmetic

References

  • De Riek, J., Calsyn, E., Everaert, I., Van Bockstaele, E., De Loose, M.: AFLP based alternatives for the assessment of distinctness, uniformity and stability of sugar beet varieties. — Theor. appl. Genet. 103:1254–1265, 2001.

    Article  Google Scholar 

  • Erschadi, S., Haberer, G., Schoniger, M., Torres-Ruiz, R.A.: Estimating genetic diversity of Arabidopsis thaliana ecotypes with amplified fragment length polymorphism (AFLP). — Theor. appl. Genet. 100: 633–640, 2000.

    CAS  Google Scholar 

  • Feyissa, T., Welander, M., Negash L.: Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica. — Biol. Plant. 51: 15–21, 2007.

    Article  CAS  Google Scholar 

  • Felsenstein, J.: Confidence limits on phylogeny: an approach using the bootstrap. — Evolution 13: 783–791, 1985.

    Article  Google Scholar 

  • Joshi, P., Dhawan, V.: Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay. — Biol. Plant. 51: 22–26, 2007.

    Article  CAS  Google Scholar 

  • Li, F.Y., Li F.G., Xing, L.J., Zhang R.G., Jiang, Y.D.: Research in the propagational technology of tissue-cultured seedlings of E. globulus pulp species. — J. Baoshan Teachers College 19: 1–3, 2000.

    Google Scholar 

  • Link, W., Dixkens, C., Singh, M., Schwall, A., Melhiger, A.E.: Genetic diversity in European and Mediterranean faba bean germplasm revealed by RAPD markers. — Theor. appl. Genet. 90: 27–32, 1995.

    Article  CAS  Google Scholar 

  • Mukherjee, A.K., Acharya, L.K., Mattagajasingh, I., Panda, P.C., Mohapatra, T., Das, P.: Molecular characterization of three Heriteria species using AFLP markers. — Biol. Plant. 47: 445–448, 2003/4.

    Article  CAS  Google Scholar 

  • Pejic, I., Ajmone-Marsan, P., Morgante, M., Kozumplick, V., Castiglioni, P., Taramino, G., Motto, M.: Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs. — Theor. appl. Genet. 97: 1248–1255, 1998.

    Article  CAS  Google Scholar 

  • Saker, M.M., Adawy, S.S., Mohamed, A.A., El-Itriby, H.A.: Monitoring of cultivar identity in tissue culture-derived date palms using RAPD and AFLP analysis. — Biol. Plant. 50: 198–204, 2006.

    Article  CAS  Google Scholar 

  • Schneider, S., Roessli, D., Excoffier, L.: Arlequin ver. 2.000: a Software for Population Genetic Data Analysis. — Genetics and Biometry Laboratory, University of Geneva, Geneva 2000.

    Google Scholar 

  • Van de Peer, Y., Watcher, R.D.: TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. — Computer Appl. BioSci. 10: 569–570, 1994.

    Google Scholar 

  • Vendrame, W.A., Kochert, G.D., Sparks, D., Wetzstein, H.Y.: Field performance and molecular evaluations of pecan trees regenerated from somatic embryogenic cultures. — J. amer. Soc. hort. Sci. 125: 542–546, 2000.

    CAS  Google Scholar 

  • Vendrame, W.A., Kochert, G., Wetzstein, H.Y.: AFLP analysis of variation in pecan somatic embryos. — Plant Cell Rep. 18: 853–857, 1999.

    Article  CAS  Google Scholar 

  • Wilhelm E.: Somatic embryogenesis in oak (Quercus spp.). — In Vitro cell dev. Biol. Plant. 36: 349–357, 2000.

    Article  CAS  Google Scholar 

  • Zhang, H.Y., Liu, X.Z., Li, T.S., Yang, Y.M.: Genetic diversity among flue-cured tobacco (Nicotiana tabacum L.) revealed by amplified fragment length polymorphism. — Bot. Stud. 47: 223–229, 2006.

    CAS  Google Scholar 

  • Zhang, H.Y., Liu, X.Z., Wei, L., Zhou, L.Y., Yang, Y.M.: Transgenic tobacco plants containing Bt and GNA genes — Biol. Plant. 51: 746–748, 2007.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Southwest Forestry College, White Dragon Temple, Kunming, Yunnan Province, 650224, P.R. China

    X. Y. Mo, X. Z. Liu, Y. M. Yang & H. Y. Zhang

  2. South China Agricultural University, Shipai, Guangzhou, Guangdong Province, 518004, P.R. China

    X. Y. Mo

  3. Leizhou Forestry Bureau, Shuiqi, Guangdong Province, 524348, P.R. China

    T. Long & H. Lin

  4. Center for Biotechnology and Bioengineering, University of Pittsburgh, Pittsburgh, PN, 15219, USA

    Z. Liu

Authors
  1. X. Y. Mo
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  2. T. Long
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  3. Z. Liu
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  4. H. Lin
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  5. X. Z. Liu
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  7. H. Y. Zhang
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Corresponding author

Correspondence to H. Y. Zhang.

Additional information

Acknowledgements: The study was supported by grants from National Basic Research Program (“973” Plan) of China (Grant No. 2003 CB 415102) and the Natural Science Foundation of Yunnan (Grant No. 2007 C 216 M).

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Mo, X.Y., Long, T., Liu, Z. et al. AFLP analysis of somaclonal variations in Eucalyptus globulus . Biol Plant 53, 741–744 (2009). https://doi.org/10.1007/s10535-009-0135-7

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  • DOI: https://doi.org/10.1007/s10535-009-0135-7

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