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Chitinases of Coffea arabica genotypes resistant to orange rust Hemileia vastatrix

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Biologia Plantarum

Abstract

Two Coffea arabica — Hemileia vastatrix incompatible interactions (I1: coffee cv. Caturra — rust race VI and I2: coffee cv S4 Agaro — rust race II) and a compatible interaction (coffee cv. Caturra — rust race II) were compared in relation to the infection process and chitinase activity. In the two incompatible interactions the fungus ceased growth in the early infection stages, while in the compatible interaction no fungus growth inhibition was observed. A high constitutive level of chitinase activity was detected in the intercellular fluid of healthy leaves. Upon infection, chitinase isoforms were more abundant in incompatible interactions than in the compatible interaction. Immunodetection showed that class I chitinases are particularly relevant in the incompatible interactions and might participate in the defence response of the coffee plants.

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Abbreviations

HR:

hypersensitive reaction

IF:

intercellular fluids

PR:

pathogenesis-related

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Authors and Affiliations

  1. IICT — Centro de Investigação das Ferrugens do Cafeeiro, Quinta do Marquês, 2784-505, Oeiras, Portugal

    L. Guerra-Guimarães, M. C. Silva, A. Loureiro & C. J. Rodrigues Jr.

  2. Faculty of Agricultural and Environmental Sciences, University of Rostock, 18059, Rostock, Germany

    C. Struck

  3. Institut de Recherche pour le Développement, UMR RPB, BP 64501-34394, Montpellier Cedex 5, France

    M. Nicole

  4. Instituto de Tecnologia Química e Biológica, Apartado 127, 2781-901, Oeiras, Portugal

    C. P. P. Ricardo

Authors
  1. L. Guerra-Guimarães
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  2. M. C. Silva
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  3. C. Struck
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  4. A. Loureiro
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  5. M. Nicole
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  6. C. J. Rodrigues Jr.
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  7. C. P. P. Ricardo
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Corresponding author

Correspondence to L. Guerra-Guimarães.

Additional information

Acknowledgments: We thank Prof. Kurt Mendgen for the facilities provided in his laboratory. The technical assistance of Ms. Cândida Monte Sousa is acknowledged.

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Guerra-Guimarães, L., Silva, M.C., Struck, C. et al. Chitinases of Coffea arabica genotypes resistant to orange rust Hemileia vastatrix . Biol Plant 53, 702–706 (2009). https://doi.org/10.1007/s10535-009-0126-8

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  • DOI: https://doi.org/10.1007/s10535-009-0126-8

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