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Antioxidant protection during ageing and senescence in transgenic tobacco with enhanced activity of cytokinin oxidase/dehydrogenase

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Biologia Plantarum

Abstract

We studied changes in physiological parameters of whole leaves and in antioxidant protection of chloroplasts during ageing and senescence of tobacco (Nicotiana tabacum L. cv. Samsun NN) leaves with enhanced cytokinin oxidase/dehydrogenase activity (CKX) or without it (WT). Old leaves of CKX plants maintained higher pigment content and photosystem 2 activity compared to WT leaves of the same age. Chloroplasts of old CKX plants showed better antioxidant capacity represented by higher superoxide dismutase, dehydroascorbate reductase and glutathione reductase activities.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbate

BSA:

bovine serum albumin

Car:

carotenoids

Chl:

chlorophyll

CKX:

transgenic tobacco with enhanced cytokinin oxidase/dehydrogenase activity

CKs:

cytokinins

DHAR:

dehydroascorbate reductase

DTNB:

5,5-dithiobis-2-nitrobenzoic acid

DTT:

dithiothreitol

FM :

maximum fluorescence

FV :

variable fluorescence

GR:

glutathione reductase

MDHAR:

monodehydroascorbate reductase

PS:

photosystem

ROS:

reactive oxygen species

SOD:

superoxide dismutase

WT:

wild type plants

XTT:

2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt

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Authors and Affiliations

  1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1, CZ-16000, Prague, Czech Republic

    D. Procházková & N. Wilhelmová

Authors
  1. D. Procházková
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  2. N. Wilhelmová
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Correspondence to D. Procházková.

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Acknowledgements: This work was supported by the Grant Agency of the Czech Republic, grant No. 522/05/P558. The authors are highly indebted Prof. Schmülling and Dr. Werner (Freie Universität Berlin, Germany) for providing the plant seeds.

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Procházková, D., Wilhelmová, N. Antioxidant protection during ageing and senescence in transgenic tobacco with enhanced activity of cytokinin oxidase/dehydrogenase. Biol Plant 53, 691–696 (2009). https://doi.org/10.1007/s10535-009-0124-x

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  • DOI: https://doi.org/10.1007/s10535-009-0124-x

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