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Effects of salicylic acid on the photosystem 2 of barley seedlings under osmotic stress

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Biologia Plantarum

Abstract

The effects of exogenous salicylic acid (SA) on photosystem 2 (PS 2) in barley (Hordeum vulgare L.) seedlings were investigated. SA pretreatment provided protection against subsequent osmotic stress. The highest protective effect of 0.25 mM SA was confirmed by determination of chlorophyll fluorescence, electrolyte leakage, malonyldialdehyde contents, PS 2 mRNAs and proteins. SA pretreatment increased reactive oxygen species (ROS), decreased net photosynthetic rate and stomatal conductance immediately, but prevented ROS accumulation during subsequent osmotic stress by activating antioxidant enzymes. Elimination of H2O2 during SA pretreatment inhibited almost all above mentioned SA effects. Therefore, SA pretreatment enhanced osmotic stress tolerance in barley seedlings mainly through ROS signals, rather than SA itself. The only SA-dependent and ROS-independent effect of exogenous SA on PS 2 was reduction of non-photochemical quenching.

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Abbreviations

DMTU:

dimethylthiourea

PS 2:

photosystem 2

ROS:

reactive oxygen species

RWC:

relative water content

SA:

salicylic acid

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Authors and Affiliations

  1. Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, 610064, P.R. China

    M. -H. Luo, S. Yuan, Y. -E. Chen, W. -J. Liu, J. -B. Du, T. Lei, M. -B. Wang & H. -H. Lin

  2. Department of Life Science and Technology, Mianyang Normal University, Mianyang, 621000, Sichuan, P.R. China

    M. -H. Luo

Authors
  1. M. -H. Luo
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  2. S. Yuan
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  3. Y. -E. Chen
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  4. W. -J. Liu
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  5. J. -B. Du
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  6. T. Lei
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  7. M. -B. Wang
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  8. H. -H. Lin
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Corresponding author

Correspondence to H. -H. Lin.

Additional information

Acknowledgments: This work was supported by the National Nature Science Foundation of China (30970214, 30670166 and 30800071), the National Key Basic Research ‘973’ Program of China (2009CB118500), and Project of Chinese Ministry of Education (108110). The first two authors contributed equally to this work.

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Luo, M.H., Yuan, S., Chen, Y.E. et al. Effects of salicylic acid on the photosystem 2 of barley seedlings under osmotic stress. Biol Plant 53, 663–669 (2009). https://doi.org/10.1007/s10535-009-0120-1

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  • DOI: https://doi.org/10.1007/s10535-009-0120-1

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