Skip to main content
SpringerLink
Log in

Overexpression of the γ-TMT gene in Codonopsis lanceolata

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

A cDNA-encoding γ-tocopherol methyltransferase (γ-TMT) from Arabidopsis thaliana was overexpressed in deoduck (Codonopsis lanceolata L.) to improve the tocopherol composition. Deoduck (T2) containing the γ-TMT transgene was produced by Agrobacterium-mediated transformation. Transgene expression was confirmed by polymerase chain reaction and RNA gel blot analysis. The transgenic plants produced more leaves than control plants. In addition, the transgenic plants showed higher levels of the CSOD, CTRX, CAPX, CNADP +-IDCH, and CSO transcripts and higher SOD-like activity compared with the control plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-benzylaminopurine

LB:

Luria Bertani

MS:

Murashige and Skoog

NAA:

α-naphthaleneacetic acid

TAE:

Tris-acetate-EDTA

References

  • Agrawala, G.K., Jwac, N.S., Iwahashid, H., Rakwalb, R.: Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling. — Gene 322: 93–103, 2003.

    Article  Google Scholar 

  • Borland, A., Elliott, S., Patterson, S.: Are the metabolic components of crassulacean acid metabolism up-regulated in responses to an increase in oxidative burden? — J. exp. Bot. 57: 319–328, 2006.

    Article  CAS  PubMed  Google Scholar 

  • Brigelius-Flohe, R., Traber, M.G.: Vitamin E: function and metabolism. — FASEB J. 13: 1145–1155, 1999.

    CAS  PubMed  Google Scholar 

  • Cho, K.S., Jang, J.E., Ryon, C., Kwon, M.: Development of transgenic plant (Codonopsis lanceolata Trautv.) harboring a Bialaphos resistance gene bar. — Korean J. Tissue Cult. 26: 281–287, 1999.

    Google Scholar 

  • Del Rio, L.A., Sandalio, L.M., Corpas, F.J.: Reactive oxygen species and reactive nitrogen species in peroxisomes. Production, scavenging, and role in cell signaling. — Plant Physiol. 141: 330–335, 2006.

    Article  PubMed  Google Scholar 

  • Demurin, Y., Skoric, D., Karlovic, D.: Genetic variability of tocopherol composition in sunflower seeds as a basis of breeding for improved oil quality. — Plant Breed. 115: 33–36, 1996.

    Article  CAS  Google Scholar 

  • Fryer, M.J.: The antioxidant effects of thylakoid vitamin E (α-tocopherol). — Plant Cell Environ. 15: 381–392, 1992.

    Article  CAS  Google Scholar 

  • Gordon, R.G., Alicia, R.V., Carmen, L.W., McIntosh, L.: Transgenic tobacco (Nicotiana tabacum L.) plants with increased expression levels of mitochondrial NADP+-dependent isocitrate dehydrogenase: evidence implicating this enzyme in the redox activation of the alternative oxidase. — Plant Cell Physiol. 45: 1413–1425, 2004.

    Article  Google Scholar 

  • Goujon, T., Ferret, V., Mila, I., Pollet, B., Ruel, K., Burlat, V., Joseleau, J.P., Barriere, Y., Lapierre, C., Jouanin, L.: Downregulation of the AtCCR1 gene in Arabidopsis thaliana: effects on phenotype lignins and cell wall degradability. — Planta 217: 218–228, 2003.

    CAS  PubMed  Google Scholar 

  • Grusak, M.A., DellaPenna, D.: Improving the nutrient composition of plants to enhance human nutrition and health. — Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 133–161, 1999.

    Article  CAS  PubMed  Google Scholar 

  • Hänsch, R., Lang, C., Rennenberg, H., Mendel, R.R.: Significance of plant sulfite oxidase. — Plant Biol. 9: 589–595, 2007.

    Article  PubMed  Google Scholar 

  • Hare, P.D., Cress, W.A., Van Staden, J.: Dissecting the roles of osmolyte accumulation during stress. — Plant Cell Environ. 21: 535–553, 1998.

    Article  CAS  Google Scholar 

  • Igamberdiev, A.U., Hill, R.D.: Nitrate, NO and haemoglobin in plant adaptation to hypoxia: an alternative to classic fermentation pathways. — J. exp. Bot. 55: 2473–2482, 2004.

    Article  CAS  PubMed  Google Scholar 

  • In, J.G., Lee, B.S., Rho, Y.D., Yu, C.Y., Yang, D.C.: Isolation and characterization of thioredoxin cDNA from Codonopsis lanceolata (S. et Z.) Trautv. — Korean J. med. Crop Sci. 13: 293–297, 2005a.

    Google Scholar 

  • In, J.G., Lee, B.S., Yi, T.H., Yu, C.Y., Yang, D.C.: Molecular cloning and characterization of type 2 metallothonein cDNA from Codonopsis lanceolata (S. et Z.) Trautv. — Korean J. med. Crop. Sci. 15: 288–292, 2005b.

    Google Scholar 

  • Jo, C., Jeong, I.Y., Kim, D.S., Son, J.H., An, B.J., Choi, J.S., Byun, M.W.: Comparison of the biological activity between a radiation-processed natural extract and a commercial counterpart for an industrial application. — Food Eng. Progr. 9: 177–181, 2005.

    Google Scholar 

  • Kholová, J., Sairam, R.K., Meena, R.C., Srivastava, G.C.: Response of maize genotypes to salinity stress in relation to osmolytes and metal-ions contents, oxidative stress and antioxidant enzymes activity. — Biol. Plant. 53: 249–256, 2009.

    Article  Google Scholar 

  • Kiffin, R., Bandyopadhyay, U., Cuervo, A.M.: Oxidative stress and autophagy. — Antioxidants Redox Sign. 8: 152–162, 2006.

    Article  CAS  Google Scholar 

  • Kim, Y.J., Seo, H.Y., Park, T.I., Baek, S.H., Shin, W.C., Kim, H.S., Kim, J.G., Choi, Y.E., Yun, S.J.: Enhanced biosynthesis of α-tocopherol in transgenic soybean by introducing γ-TMT gene. — J Plant Biotechnol. 7: 1–7, 2005.

    Google Scholar 

  • Laloi, C., Mestres-Ortega, D., Marco, Y., Meyer, Y., Reichheld, J.P.: The arabidopsis cytosolic thioredoxin h5 gene induction by oxidative stress and its W-box-mediated response to pathogen elicitor. — Plant Physiol. 134: 1006–1016, 2004.

    Article  CAS  PubMed  Google Scholar 

  • Lang, C., Pork, J., Wirtz, M., Hell, R., Herschbach, C., Kreuzwieser, J., Rennenberg, H., Mendel, R.R., Hansch, R.: Sulphite oxidase as key enzyme for protecting plants against sulphur dioxide. — Plant Cell Environ. 30: 447–455, 2007.

    Article  CAS  PubMed  Google Scholar 

  • McKersie, B.D., Hoekstra, F.A., Krieg, L.C.: Differences in the susceptibility of plantmembrane lipids to peroxidation. — Biochim. biophys. Acta 1030: 119–126, 1990.

    Article  CAS  PubMed  Google Scholar 

  • Min, S.R., Yang, S.G., Liu, J.R., Choi, P.S., Soh, W.Y.: High frequency somatic embryogenesis and plant regeneration in tissue cultures of Codonopsis lanceolata. — Plant Cell Rep. 10: 621–623, 1992.

    Article  Google Scholar 

  • Møller, I.M.: Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. — Annu. Rev.Plant Physiol. Plant mol. Biol. 52: 561–591, 2001.

    Article  PubMed  Google Scholar 

  • Noctor, G.: Metabolic signaling in defence and stress: the central roles of soluble redox couples. — Plant Cell Environ. 29: 409–425, 2006.

    Article  CAS  PubMed  Google Scholar 

  • Piquemal, J., Lapierre, C., Myton, K., O’Connell, A., Schuch, W., Grima-Pettenati, J., Boudet, A.M.: Downregulation of cinnamoyl-CoA reductase induces significant changes of lignin profiles in transgenic tobacco plants. — Plant J. 13: 71–83, 1998.

    Article  CAS  Google Scholar 

  • Rimm, E.B., Stampfer, M.J., Ascherio, A., Giovannucci, E., Colditz, G.A., Willett, W.C.: Vitamin E consumption and the risk of coronary heart disease in men. — N. Engl. J. Med. 328: 1450–1456, 1993.

    Article  CAS  PubMed  Google Scholar 

  • Rogers, L.A., Campbell, M.M.: The genetic control of lignin deposition during plant growth and development. — New Phytol. 164: 17–30, 2004.

    Article  CAS  Google Scholar 

  • Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y., Yoshimura, K.: Regulation and function of ascorbate peroxidase isoenzymes: — J. exp. Bot. 53: 1305–1319, 2002.

    Article  CAS  PubMed  Google Scholar 

  • Shin, J.H., Park, M.C.: Introduction of RAG25 gene into Codonopsis lanceolata by Agrobacterium tumefaciens. — Korean J. Plant Tissue Cult. 27: 491–496, 2000.

    Google Scholar 

  • Shintani, D., DellaPenna, D.: Elevating the vitamin E content of plants through metabolic engineering. — Science 282: 2098–2100, 1998.

    Article  CAS  PubMed  Google Scholar 

  • Van Eenennaam, A.L., Lincoln, K, Durrett, T.P., Valentin, H.E., Shewmaker, C.K., Thorne, G.M., Jiang, J., Baszis, S.R., Levering, C.K., Aasen, E.D., Hao, M. Stein, J.C., Norris, S.R., Last, R.L.: Engineering vitamin E content: from Arabidopsis mutant to soy oil. — Plant Cell 15: 3007–3019, 2003.

    Article  PubMed  Google Scholar 

  • Venkata, S.T., Kim, Y.H., Isabelle, A.K., Randy, D.D., Kim, K.H., Glenn, B.C.: Increased α-tocopherol content in soybean seed overexpressing the Perilla frutescens γ -tocopherol methyltransferase gene. — Plant Cell Rep. 26: 61–70, 2007.

    Google Scholar 

  • Yi, S.Y., Kim, J.H., Joung, Y.H., Lee, S., Kim, W.T., Yu, S.H., Choi, D.: The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis. — Plant Physiol. 136: 2862–2874, 2004.

    Article  CAS  PubMed  Google Scholar 

  • Yusuf, M.A., Sarin, N.B.: Antioxidant value addition in human diets: genetic transformation of Brassica juncea with γ-TMT gene for increased α-tocopherol content. — Transgenic Res. 16: 109–113, 2007.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bioherb Research Institute, Kangwon National University, Chunchon, 200-701, South Korea

    E. S. Seong, E. J. Goh, M. J. Kim & C. Y. Yu

  2. Department of Applied Life Science, Konkuk University, Seoul, 143-701, South Korea

    B. K. Ghimire & I. M. Chung

  3. Department of Herbal Medicine Resource, Kangwon National University, Samcheok, 245-711, South Korea

    J. D. Lim

Authors
  1. E. S. Seong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. K. Ghimire
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. J. Goh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. D. Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. J. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. I. M. Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. C. Y. Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. Y. Yu.

Additional information

Acknowledgements: This work was supported by the Research Grant from Kangwon National University. And, this work was published by partial financial support of Bioherb Research Institute and the Agriculture & Life Sciences Research Institute, Kangwon National University, Republic of Korea.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Seong, E.S., Ghimire, B.K., Goh, E.J. et al. Overexpression of the γ-TMT gene in Codonopsis lanceolata . Biol Plant 53, 631–636 (2009). https://doi.org/10.1007/s10535-009-0115-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-009-0115-y

Additional key words

Search

Navigation