Agrobacterium-mediated genetic transformation of plants: The role of host
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- Karami, O., Esna-Ashari, M., Karimi Kurdistani, G. et al. Biol Plant (2009) 53: 201. doi:10.1007/s10535-009-0041-z
Agrobacterium-mediated genetic transformation is the most widely used technology to obtain overexpression of recombinant proteins in plants. Molecular events that occur within Agrobacterium during interactions with host plants have been studied extensively, and now we have a reasonable understanding the key factors involved in the regulation of T-DNA nuclear import and genomic integration. By contrast, very little is known about the events that take place in the host cells during genetic transformation by Agrobacterium. Here, we describe the plant-related factors including genotype, genes, proteins, competency of target tissues and phenolic compounds that participate in Agrobacterium-mediated genetic transformation and discuss their possible roles in this process. Because Agrobacterium probably adapts existing cellular processes for its life cycle, identifying the processes in host cells during Agrobacterium infection might contribute to better understanding of basic biological processes as cell communication, intracellular transport and DNA repair and recombination as well as to expanding the host range of Agrobacterium as a genetic engineering tool.
Additional key wordsT-DNA vir gene VIR protein
mitogen-activated protein kinase
nuclear localization signal
pathogen associated molecular patterns
pattern recognition receptors
systemic acquired resistance
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