Biologia Plantarum

, Volume 53, Issue 1, pp 75–84 | Cite as

Waterlogging induced oxidative stress and antioxidant activity in pigeonpea genotypes

  • D. Kumutha
  • K. Ezhilmathi
  • R. K. Sairam
  • G. C. Srivastava
  • P. S. Deshmukh
  • R. C. Meena
Original Papers

Abstract

The objective of this study was to examine the role of antioxidant enzymes in waterlogging tolerance of pigeonpea (Cajanus cajan L. Halls) genotypes ICP 301 (tolerant) and Pusa 207 (susceptible). Waterlogging resulted in visible yellowing and senescence of leaves, decrease in leaf area, dry matter, relative water content and chlorophyll content in leaves, and membrane stability index in roots and leaves. The decline in all parameters was greater in Pusa 207 than ICP 301. Oxidative stress in the form of superoxide radical, hydrogen peroxide and thiobarbituric acid reactive substances (TBARS) contents initially decreased, however at 4 and 6 d of waterlogging it increased over control plants, probably due to activation of DPI-sensitive NADPH-oxidase. Antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, glutathione reductase and catalase also increased under waterlogging. The comparatively greater antioxidant enzyme activities resulting in less oxidative stress in ICP 301 could be one of the factor determining its higher tolerance to flooding as compared to Pusa 207. This study is the first to conclusively prove that waterlogging induced increase in ROS is via NADPH oxidase.

Additional key words

anoxia ascorbate peroxidase Cajanus cajan catalase glutathione reductase hydrogen peroxide hypoxia oxidative stress superoxide radical superoxide dismutase 

Abbreviations

APX

ascorbate peroxidase

CAT

catalase

Chl

chlorophyll

DAA

days after anthesis

DPI

diphenylene iodonium chloride

GR

glutathione reductase

MSI

membrane stability index

ROS

reactive oxygen species

RWC

relative water content

SOD

superoxide dismutase

TBARS

thiobarbituric acid reactive substances

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • D. Kumutha
    • 1
  • K. Ezhilmathi
    • 1
  • R. K. Sairam
    • 1
  • G. C. Srivastava
    • 1
  • P. S. Deshmukh
    • 1
  • R. C. Meena
    • 1
  1. 1.Indian Agricultural Research InstituteDivision of Plant PhysiologyNew DelhiIndia

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