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Biologia Plantarum

, Volume 52, Issue 4, pp 713–717 | Cite as

Efficient regeneration of Eucommia ulmoides from hypocotyl explant

  • R. Chen
  • S. Namimatsu
  • Y. Nakadozono
  • T. Bamba
  • Y. Nakazawa
  • K. Gyokusen
Brief Communication

Abstract

A rapid and efficient method for the regeneration of Eucommia ulmoides Oliver has been developed. The ability of hypocotyl segments to produce adventitious buds varied depending upon their position, seedling age and culture medium. The most adventitious buds were induced from the hypocotyl segments near to the root of 2-week-old seedlings on the Murashige and Skoog (MS) basal medium supplemented with 10 µM benzylaminopurine (BAP). However, the MS medium with 6 µM BAP and 6 µM gibberellic acid (GA3) was most suitable for shoot multiplication and further growth. Shoots longer than 2 cm cultured on the half-strength MS medium supplemented with 0.5 µM naphthaleneacetic acid (NAA) produced a maximum number of roots per shoot. Regenerated plantlets could be successfully acclimatized.

Additional key words

auxin cytokinin gibberellic acid histological analysis shoot multiplication and elongation 

Abbreviations

BAP

6-benzylaminopurine

GA3

gibberellic acid

IAA

3-indoleacetic acid

IBA

3-indolebutyric acid

MS medium

Murashige and Skoog medium (1962)

NAA

naphthaleneacetic acid

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References

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • R. Chen
    • 1
  • S. Namimatsu
    • 1
  • Y. Nakadozono
    • 1
  • T. Bamba
    • 1
  • Y. Nakazawa
    • 1
  • K. Gyokusen
    • 2
  1. 1.Technical Research InstituteHitachi Zosen CorporationOsakaJapan
  2. 2.Department of Forest and Forest Products Sciences, Faculty of AgricultureKyushu UniversityFukuokaJapan

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