Biologia Plantarum

, Volume 52, Issue 3, pp 538–542 | Cite as

An improved protocol for micropropagation of elite genotypes of Simmondsia chinensis (Link) Schneider

Brief Communication


An efficient micropropagation protocol was developed for elite male and female genotypes of Simmondsia chinensis using nodal segments. Bud initiation was found to be best on Murashige and Skoog’s (MS) medium supplemented with 4.44 µM 6-benzylaminopurine (BAP) and 88.8 µM adenine. Upon sub-culture, 10–15 shoots per explant were obtained when 4.44 µM BAP and 74.0 µM adenine were incorporated in the medium. Increase in KNO3 concentration in the medium improved shoot multiplication rate and in vitro flowering in 20 % of male cultures. Elongated shoots were harvested, pulse treated for 48 h on liquid medium supplemented with 49.0 µM indole-3-butyric acid, 5.40 µM α-naphthaleneacetic acid and 5.71 µM indole-3-acetic acid for root induction and rooting (92 %) was achieved on hormonal free half-strength MS medium supplemented with 1.37 µM chlorogenic acid, 1 % activated charcoal and 2 % sucrose. After successful hardening, plantlets were transferred to greenhouse with 99 % establishment.

Additional key words

female flowering jojoba male multiplication vitrification 





indole-3-acetic acid


indole-3-butyric acid

MS medium

Murashige and Skoog’s medium


α-naphthaleneacetic acid


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  1. Alcaraz, M.L., Ayla-Rocha, B.: Asexual reproduction of Jojoba by layering.-Hort. Sci. 17: 893–96, 1982.Google Scholar
  2. Babic, V., Nescovic, M.: Propagation of three blackberry cultivars from small apical bud in vitro.-J. hort. Sci. 59: 183–185, 1984.Google Scholar
  3. Chaturvedi, H.C., Sharma, M.: In vitro production of cloned plants of Jojoba (Simmondsia chinensis (Link) Schneider) through shoot proliferation in long term culture.-Plant Sci. 63: 199–207, 1989.CrossRefGoogle Scholar
  4. Collet, G.F.: Improvement to induce rooting of fruit trees in vitro.-Acta Hort. 227: 318–324. 1988.Google Scholar
  5. Driver, J.A., Kuniyuki, A.H.: In vitro propagation of paradox walnut rootstock.-Hort. Sci. 19: 507–509, 1984.Google Scholar
  6. Jacoboni, A., Standarti, A.: Tissue culture of jojoba (Simmondsia chinensis Link).-Acta Hort. 212: 557–560, 1987.Google Scholar
  7. Kaliamoorthy, S., Naidoo, G., Achar, P.: Micropropagation of Harpagophytum procumbens.-Biol. Plant. 52: 191–194, 2008.CrossRefGoogle Scholar
  8. Lee, C.W., Palzkill, D.A.: Propagation of jojoba by single node cuttings.-Hort. Sci 19: 841–842, 1984.Google Scholar
  9. Llorente, B.E., Apostolo, N.M.: Effect of different growth regulators and genotype on in vitro propagation of jojoba.-New Zealand J. of Crop and Hort. Sci. 26: 55–62, 1998.Google Scholar
  10. Mandani, A., Lee, C.W., Hogan, L.: In vitro propagation of Simmondsia chinensis via shoot tip culture.-Hort. Sci. 13: 35–37, 1978.Google Scholar
  11. Mills, D., Wenkart, S., Benzioni, A.: Micropropagation of Simmondsia chinensis (jojoba).-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Vol. 40. Pp. 380–939. Springer-Verlag, Berlin-Heidelberg 1997.Google Scholar
  12. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. plant. 15: 473–497, 1962.CrossRefGoogle Scholar
  13. Peare, H.L.: Experiments with growth controlling substances. II. Response of the fruit-tree cuttings to treatment with synthetic root forming substances.-Rep. East Malling Res. Sta. 21: 157–166, 1938.Google Scholar
  14. Rao, M.S., Purohit, S.D.: In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd.-Biol. Plant. 50: 501–506, 2006.CrossRefGoogle Scholar
  15. Roussos, P.A., Tolia-Marioli, A., Pontikis, C.A., Kotsias, D.: Rapid multiplication of jojoba seedlings by in vitro culture.-Plant Cell Tissue Organ Cult. 57: 133–137, 1999.CrossRefGoogle Scholar
  16. Selvaraj, N., Vasudevan, A., Manikavasagam, M., Ganpathi, A.: In vitro organogenesis and plant formation in cucumber.-Biol. Plant. 50: 123–126, 2006.CrossRefGoogle Scholar
  17. Torrey, J.G.: Root hormones and plant growth.-Annu. Rev. Plant Physiol. 27: 435–459, 1976.CrossRefGoogle Scholar
  18. Tyagi, R.K., Prakash, S.S.: Genotype-and sex-specific protocol for in vitro micropropagation and medium-term conservation of jojoba.-Biol. Plant. 48: 119–123, 2004.Google Scholar
  19. Yermanos, D.M.: Jojoba-a crop whose time has come.-California Agr. 33: 4–9 and 10–11, 1979.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Discipline of Phytosalinity, Central Salt and Marine Chemicals Research Institute, BhavnagarGujaratIndia

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