Skip to main content
Log in

Genetic analysis of fertility-restorer genes in rice

  • Original Papers
  • Published:
Biologia Plantarum


Wild-abortive (WA), Honglian (HL) and Baro-II (BT) are three important cytoplasmic male sterility (CMS) types in rice. It is essential to investigate genetic mode and allelism of fertility restorer (Rf) genes and the relationship between Rf and CMS. Fertility of the all test-cross F1 plants shows that the restorer-maintainer relationship is similar for HL-CMS and BT-CMS, while that is variance for WA-CMS and HL-CMS (or BT-CMS), respectively. Genetic analysis of Rf genes indicates that HL-or BT-CMS are controlled by single dominant Rf gene and WA-CMS is controlled by one or two pairs of dominant Rf genes, which reflects the characters of the gametophytic and sporophytic restoration CMS type. It is concluded that there are at least three Rf loci in different accessions with Rf genes for each CMS type.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others





cytoplasmic male sterility



Rf :

fertility restorer




  • Akagi, H., Nakamura, A., Yokozeki-Misono, Y., Inagaki, A., Takahashi, H., Mori, K., Fujimura, T.: Positional cloning of the rice Rf-1 gene, a restorer of BT-type cytoplasmic male sterility that encodes a mitochondria-targeting PPR protein.-Theor. appl. Genet. 108: 1449–1457, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Bellaoui, M., Grelon, M., Pelletier, G., Budar, F.: The restorer Rfo gene acts post-translationally on the stability of the ORF138 Ogura CMS-associated protein in reproductive tissues of rapeseed cybrids.-Plant. mol. Biol. 40: 893–902, 1999.

    Article  PubMed  CAS  Google Scholar 

  • Dalmacio, R.D., Ishii, T.: Identification and transfer of a new cytoplasmic male sterility source from Oryza perennis into rice (O. sativa).-Euphytica 82: 221–225, 1995.

    Article  Google Scholar 

  • Gabay-Laughnan, S., Laughnan, J.R.: Male sterility and restorer genes in maize.-In: Freeling, M., Walbot, V. (ed.): The Maize Handbook. Pp. 418–423. Springer, New York-Berlin-Heidelberg 1994.

    Google Scholar 

  • Komori, T., Yamamoto, T., Takemori, N., Kashihara, M., Matsushima, H., Nitta, N.: Fine genetic mapping of the nuclear gene, Rf-1, that restores the BT-type cytoplasmic male sterility in rice (Oryza sativa L.) by PCR-based markers.-Euphytica 129: 241–247, 2003.

    Article  CAS  Google Scholar 

  • Hoan, N.T., Sarma, N.P., Siddiq, E.A.: Wide hybridization for diversification of CMS in rice.-Int. Rice Res. Notes 23: 5–6, 1998.

    Google Scholar 

  • Li, J.M., Yuan, L.P.: Hybrid rice: genetics, breeding, and seed production.-Plant Breed. Rev. 17: 15–158, 2000.

    CAS  Google Scholar 

  • Li, S.Q., Yang, G.H., Li, S.B., Li, Y.S., Chen, Z.Y., Zhu, Y.G.: Distribution of fertility-restorer gene for Wild-abortive and Honglian CMS lines of rice in the AA genome species of genus Oryza.-Ann. Bot. 96: 461–466, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Liu, X.Q., Xu, X., Tan, Y.P., Li, S.Q., Hu, J., Huang, J.Y., Yang, D.C., Li, Y.S., Zhu, Y.G.: Inheritance and molecular mapping of two fertility-restoring loci for Honglian gametophytic cytoplasmic male sterility in rice (Oryza sativa L.).-Mol. gen. Genomics 271: 586–594, 2004.

    Article  CAS  Google Scholar 

  • Pradhan, S.B., Jachuck, P.J.: Development of new cytoplasmic source of male sterile lines in rice for shallow lowland.-Euphytica 111: 43–46, 2000.

    Article  Google Scholar 

  • Rao, Y.S.: Cytohistology of cytoplasmic male sterile lines in hybrid rice.-In: Smith, W.H., Bostian, L.R., Cervantes, E.P. (ed.): Hybrid Rice. Pp. 115–128. IRRI, Manila 1988.

    Google Scholar 

  • Rao, P.V.: Statistical Research Methods in the Life Sciences.-Duxbury Press, Duxbury-New York 1998.

    Google Scholar 

  • Tan, X.L., Trangoonrang, S.: Genetic analysis of rice CMS-WA fertility restoration based on QTL mapping.-Theor. appl. Genet. 96: 994–999, 1998.

    Article  Google Scholar 

  • Touzet, P., Budar, F.: Unveiling the molecular arms race between two conflicting genomes in cytoplasmic male sterility?-Trends Plant. Sci. 9: 568–570, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Van Damme, J.M.M., Hundscheid, M.P.J., Ivanovic, S., Koelewijn, H.P.: Multiple CMS-restorer gene polymorphism in gynodioecious Plantago coronopus.-Heredity 93: 175–181, 2004.

    Article  PubMed  Google Scholar 

  • Wang, Z.H., Zou, Y.J., Li, X.Y., Zhang, Q.Y., Chen, L.T., Wu, H., Su, D.H., Chen, Y.L., Guo, J.X., Luo, D., Long, Y.M., Yao, F.Y., Xu, C.G., Yu, S.B., Li, J.X., Gao, Y.J., Li, X.H., Zhang, Q.: Mapping and genetic analysis of two fertility restorer loci in wild-abortive cytoplasmic male sterility system of rice (Oryza sativa L.).-Euphytica 98: 183–187, 1997.

    Article  Google Scholar 

  • Zhu, Y.G.: Studies on rice male sterility and its restoration.-In: Zhu, Y.G., Li, Y.Q., Wang, M.Q. (ed.): Biology of Male Sterility in Rice. Pp. 398–438. Wuhan University Press, Wuhan 2000.

    Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Y. G. Zhu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tan, Y.P., Li, S.Q., Wang, L. et al. Genetic analysis of fertility-restorer genes in rice. Biol Plant 52, 469–474 (2008).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:

Additional key words