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Cyclic somatic embryogenesis and efficient plant regeneration from callus of safflower

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Efficient plant regeneration through somatic embryogenesis was established for safflower (Carthamus tinctorius L.) cv. NARI-6. Embryogenic calli were induced from 10 to 17-d-old cotyledon and leaf explants from in vitro seedlings. High frequency (94.3 %) embryogenic callus was obtained from cotyledon explants cultured on Murashige and Skoog’s germination (MSG) basal medium supplemented with thidiazuron, 2-isopentenyladenine and indole-3-butyric acid. Primary, secondary and cyclic somatic embryos were formed from embryogenic calli in a different media free of plant growth regulators, however, 100 % cyclic somatic embryogenesis was obtained from cotyledon derived embryogenic calli cultured on MSG. Somatic embryos matured and germinated in quarter-strength MSG medium supplemented with gibberellic acid. Cotyledons with root poles or non root poles were converted to normal plantlets and produced adventitious roots in rooting medium. Rooted plants were acclimatized and successfully transferred to the field.

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Abbreviations

B5 :

Gamborg medium

CDEC:

cotyledon derived embryogenic calli

2,4-D:

2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid

IBA:

indole-3-butyric acid

2-ip:

2-isopentenyladenine

KN:

kinetin

LDEC:

leaf derived embryogenic calli

MSG medium:

Murashige and Skoog’s germination medium

NAA:

naphthalene-acetic acid

PCD:

programmed cell death

PEM:

proembryogenic mass

SH:

Schenk and Hildebrandt

SE:

somatic embryogenesis

TDZ:

thidiazuron (1-phenyl-3-(1,2,3-thidiazol-5-yl)-urea

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Authors and Affiliations

  1. Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    J. Vijaya Kumar & B. D. Ranjitha Kumari

  2. CICY, UBMMP, Merida, Yucatan, CP 97200, Mexico

    E. Castaño

Authors
  1. J. Vijaya Kumar
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  2. B. D. Ranjitha Kumari
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  3. E. Castaño
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Correspondence to B. D. Ranjitha Kumari.

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Vijaya Kumar, J., Ranjitha Kumari, B.D. & Castaño, E. Cyclic somatic embryogenesis and efficient plant regeneration from callus of safflower. Biol Plant 52, 429–436 (2008). https://doi.org/10.1007/s10535-008-0087-3

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  • DOI: https://doi.org/10.1007/s10535-008-0087-3

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