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Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer

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Biologia Plantarum

Abstract

Ex vitro transfer is often stressful for in vitro grown plantlets. Water stress and photoinhibition, often accompanying the acclimatization of in vitro grown plantlets to ex vitro conditions, are probably the main factors promoting production of reactive oxygen species (ROS) and in consequence oxidative stress. The extent of the damaging effects of ROS depends on the effectiveness of the antioxidative systems which include low molecular mass antioxidants (ascorbate, glutathione, tocopherols, carotenoids, phenols) and antioxidative enzymes (superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase, monodehydroascorbate reductase, dehydroascorbate reductase). This review is focused on ROS production and development of antioxidative system during in vitro growth and their further changes during ex vitro transfer.

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Abbreviations

ABA:

abscisic acid

APX:

ascorbate peroxidase (EC 1.11.1.11)

Car:

carotenoids

CAT:

catalase

DHAR:

dehydroascorbate reductase (EC 1.8.5.1)

GA3 :

gibberellic acid

GR:

glutathione reductase (EC 1.6.4.2)

LOX:

lipoxygenase (EC 1.13.11.12)

MDA:

malondialdehyde

MDHAR:

monodehydroascorbate reductase (EC 1.6.5.4)

PEG:

polyethylene glycol

POX:

peroxidase (EC 1.11.1.7)

ROS:

reactive oxygen species

SA:

salicylic acid

SOD:

superoxide dismutase (EC 1.15.1.1)

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Baťková, P., Pospíšilová, J. & Synková, H. Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer. Biol Plant 52, 413–422 (2008). https://doi.org/10.1007/s10535-008-0085-5

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