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Glutathione and phytochelatin contents in tomato plants exposed to cadmium

Abstract

The effect of cadmium on growth and contents of glutathione (GSH) and phytochelatins (PCs) were investigated in roots and leaves of tomato plants (Lycopersicon esculentum Mill. cv. 63/5 F1). The accumulation of Cd increased with external Cd concentrations and was considerably higher in roots than in leaves. Dry mass production decreased under Cd treatment especially in leaves. In both roots and leaves, exposure to Cd caused an appreciable decline in GSH contents and increase in PCs synthesis proportional to Cd concentrations in the growth medium. At the same Cd concentration, PCs production was higher in roots than in leaves. The implication of glutathione in PC synthesis was strongly suggested by the use of buthionine sulfoximine (BSO). The major fraction of Cd accumulated by tomato roots was in the form of a Cd-PCs complex.

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Abbreviations

BSO:

buthionine sulfoximine

GSH:

glutathione

NPT:

nonprotein thiol

PCs:

phytochelatins

TBARS:

thiobarbituric acid reactive substances

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Correspondence to W. Ben Ammar.

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Ben Ammar, W., Mediouni, C., Tray, B. et al. Glutathione and phytochelatin contents in tomato plants exposed to cadmium. Biol Plant 52, 314–320 (2008). https://doi.org/10.1007/s10535-008-0065-9

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  • DOI: https://doi.org/10.1007/s10535-008-0065-9

Additional key words

  • buthionine sulfoximine
  • complex Cd-PC
  • Lycopersicon esculentum