Abstract
A method for plant regeneration in Robinia pseudoacacia L. from cell suspension culture was established. Non regenerative friable callus from hypocotyls and cotyledon explants from in vitro raised seedling induced on solid Murashige and Skoog (MS) medium supplemented with 0.05 mg dm−3 2,4-dichlorophenoxyacetic acid (2,4-D) was used for initiation of cell suspension cultures on same MS medium but without agar. Single cells were isolated after 3 d and the optimum cell density was 1–3 × 104 cells per cm3 of the liquid MS medium. Plating efficiency was 29.6 % and callus formed within 4 weeks was subcultured and transferred to solid MS medium supplemented with 0.6 mg dm−3 benzyladenine (BA) along with 0.05 mg dm−3 α-naphthalene-1-acetic acid (NAA) for the induction of adventitious bud primordia. The shoots developed were isolated and re-cultured on MS medium containing 0.6 mg dm−3 BA. These microshoots after dipping in 1–2 cm3 of 10 mg dm−3 indole-3-butyric acid (IBA) for 24 h in dark were cultured on half strength solid MS medium supplemented with 0.05 % charcoal and showed 80–82 % rooting within 4 weeks.
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Abbreviations
- BA:
-
benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- IBA:
-
indole-3-butric acid
- MS:
-
medium of Murashige and Skoog (1962)
- NAA:
-
α-naphthalene-1-acetic acid
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Kanwar, K., Kaushal, B., Abrol, S. et al. Plant regeneration in Robinia pseudoacacia from cell suspension cultures. Biol Plant 52, 187–190 (2008). https://doi.org/10.1007/s10535-008-0042-3
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DOI: https://doi.org/10.1007/s10535-008-0042-3