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Headspace-SPME of in vitro shoot-cultures and micropropagated plants of Lavandula viridis

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

In this work the volatiles emitted from in vitro shoot-cultures and micropropagated plants of Lavandula viridis L’Hér. were characterized and compared with those obtained from the field-grown mother-plant, using headspace solid phase micro-extraction following by capillary gas chromatography coupled to mass spectrometry (HS-SPME-GC/MS). The headspace composition consisted mainly in oxygenated monoterpenes (66.7–79.2 %), where the major constituents emitted by the mature field-grown mother-plant, in vitro shoot-cultures and micropropagated plants were 1,8-cineole (74.0, 51.9 and 57.8 %) and camphor (2.9, 15.3 and 8.7 %), respectively. The headspace of in vitro shoot-cultures and micropropagated plants showed greater amount of α-pinene, camphene, β-pinene, β-selinene and selina-3,7(11)-diene, when compared with the field-grown mother-plant.

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Abbreviations

BA:

benzyladenine

GC/MS:

gas chromatography coupled to mass spectrometry

FP:

field-grown mother plant

InV:

in vitro shoot cultures

MP:

micropropagated plants

MS:

Murashige and Skoog medium

SPME:

solid phase microextraction

References

  • Adams, R.P: Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectrometry.-Allured Publishing Corporation, Carol Stream 2001.

    Google Scholar 

  • Ahuja, A., Bakshi, S.K., Sharma, S.K., Tapa, R.K., Agarwal, S.G., Kichlu, S.K., Paul, R., Kaul, M.K.: Production of volatile terpenes by proliferating shoots and micropropagated plants of Santolina chamaecyparissus L. (cotton lavender).-Flavour Fragrance J. 20: 403–406, 2005.

    Article  CAS  Google Scholar 

  • Arikat N, Jawad F, Karam N, Shibli R.: Micropropagation and accumulation of essential oils in wild sage (Salvia fruticosa Mill.).-Sci. Hort. 100: 193–202, 2004.

    Article  CAS  Google Scholar 

  • Ballabeni, V., Tognoli, M., Chiavarini, M., Impicciatore, M., Bruni, R., Bianchi, A., Barocelli, E.: Novel antiplatelet and antithrombotic activities of essential oil from Lavandula hybrida Reverchon “grosso”.-Phytomedicine 11: 596–601, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Barocelli, E., Calcina, F., Chiavarini, M.M., Impicciatore, M., Bruni, R., Bianchi, A., Ballabeni, V.: Antinociceptive and gastroprotective effects of inhaled and orally administered Lavandula hybrida Reverchon “Grosso” essential oil.-Life Sci. 76: 213–223, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Bertoli, A., Pistelli, L., Morelli, I., Fraternale, D., Giamperi, L., Ricci, D.: Volatile constituents of micropropagated plants of Bupleurum fruticosum L.-Plant Sci. 167: 807–810, 2004.

    Article  CAS  Google Scholar 

  • Demirci, B., Demirci, F., Baser, K.H.C.: Headspace-SPME and hydrodistillation of two fragrant Artemisia sp.-Flavour Fragrance J. 20: 395–398, 2005.

    Article  CAS  Google Scholar 

  • Dias, M.C., Almeida, R., Romano, A.: Rapid clonal multiplication through in vitro axillary shoot proliferation of Lavandula viridis L’Hér.-Plant Cell Tissue Organ Cult. 68: 99–102, 2002.

    Article  CAS  Google Scholar 

  • Flamini, G., Cioni, P., Morelli, I.: Composition of the essential oils and in vivo emission of four Lamium species from Italy: L. purpureum, L. hybridum, L. bifidum and L. amplexicaule.-Food Chem. 91: 63–65, 2005.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture.-Physiol. Plant. 15: 473–479, 1962.

    Article  CAS  Google Scholar 

  • Nogueira, J.M.F., Romano, A.: Essential oils from micropropagated plants of Lavandula viridis.-Phytochem. Anal. 13: 4–7, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Palá-Paul, J., Brophy, J., Goldsack, R., Fontaniella, B.: Analysis of the volatile components of Lavandula canariensis (L.) Mill., a Canary Islands endemic species, growing in Australia.-Biochem. Syst. Ecol. 32: 55–62, 2004.

    Article  Google Scholar 

  • Santos, F.A., Rao, V.S.: 1,8-cineole, a food flavoring agent, prevents ethanol-induced gastric injury in rats.-Digest. Dis. Sci. 46: 331–337, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Sanz, J., Soria, A., García-Vallejo, M.: Analysis of volatile components of Lavandula luisieri L. by direct thermal desorption-gas chromatography-mass spectrometry.-J. Chromatogr. A 1024: 139–146, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Sudria, C., Palazón, J., Cusidó, R., Bonfill, M., Piñol, M.T., Morales, C.: Effect of benzyladenine and indolebutyric acid on ultrastructure, glands formation, and essential oil accumulation in Lavandula dentata plantlets.-Biol. Plant. 44: 1–6, 2001.

    Article  CAS  Google Scholar 

  • Sudria, C., Pinol, M.T., Palazon, J., Cusido, R.M., Vila, R., Morales, C., Bonfill, M., Canigueral, S.: Influence of plant growth regulators on the growth and essential oil content of cultured Lavandula dentata plantlets.-Plant Cell Tiss. Org. Cult. 58: 177–184, 1999.

    Article  CAS  Google Scholar 

  • Tawfik, A.A., Read, P.E., Cuppett, S.L.: Stimulation of growth and monoterpene production of sage (Salvia officinalis) by benzyladenine in vitro.-Plant Growth Regul. 20: 200–206, 1992.

    CAS  Google Scholar 

  • Tsuro, M., Inoue, M., Kameoka, H.: Variation in essential oil components in regenerated lavender (Lavandula vera DC) plants.-Sci. Hort. 88: 309–317, 2001.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    S. Gonçalves, R. Almeida, L. Custódio & A. Romano

  2. Departamento de Química e Bioquímica e Centro de Ciências Moleculares e Materiais, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, Ed. C8, 1749-016, Lisboa, Portugal

    H. Serra & J. M. F. Nogueira

Authors
  1. S. Gonçalves
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  2. H. Serra
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  3. J. M. F. Nogueira
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  4. R. Almeida
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  5. L. Custódio
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  6. A. Romano
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Corresponding author

Correspondence to A. Romano.

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Gonçalves, S., Serra, H., Nogueira, J.M.F. et al. Headspace-SPME of in vitro shoot-cultures and micropropagated plants of Lavandula viridis . Biol Plant 52, 133–136 (2008). https://doi.org/10.1007/s10535-008-0027-2

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  • DOI: https://doi.org/10.1007/s10535-008-0027-2

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