Abstract
Fourteen efficient inter-simple sequence repeat (ISSR) primers were screened and optimized for detecting the genetic diversity in wild populations of Glycyrrhiza uralensis Fisch. By using these primers, 249 polymorphic bands out of a total of 270 (92.2 %) were generated from 70 individuals of 4 wild G. uralensis populations sampled from Inner Mongolia Province of China. Nei’s gene diversity (h) and Shannon index (I) calculated from the data matrix of the ISSR phenotypes revealed a high level of genetic diversity with h = 0.268 and I = 0.415 within this plant. Analysis of molecular variation (AMOVA) showed that most of the genetic variation (81 %) occurred within the populations, whereas the variance among populations was only 19 %. The UPGMA tree based on Nei’s unbiased genetic diversity illustrated that populations from Bulage and Bayanwusu were genetically close related, while the population from Shanghaimiao was found to be the most diverse from the other three. The high genetic diversity implies that the wild resources of this species could be restored soon if an appropriate and efficient protection strategy was employed. Our results also provided an optimized method for evaluating genetic diversity of G. uralensis using ISSR markers which was useful for further investigation.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- CTAB:
-
cetyltrimethylammonium bromide
- ISSR:
-
inter-simple sequence repeat
- PCR:
-
polymerase chain reaction
References
Hamrick, J.L., Godt, M.J.W.: Allozyme diversity in plant species.-In: Brown, A.H.D., Clegg, M.T., Kahler, A.L., Weir, B.S. (ed.): Plant Population Genetics, Breeding and Genetic resources. Pp. 43–46. Sinauer Associates, Massachusetts 1989.
Hayashi, H., Inoue, K., Ozaki, K., Watanabe, H.: Comparative analysis of ten strains of Glycyrrhiza uralensis cultivated in Japan.-Biol. pharm. Bull. 28: 1113–1116, 2005.
Miller, M.P.: AMOVA-PREP, a Program for the Preparatition of AMOVA Input Files from Dominant Marker Raw Data (Release 1.01).-Dep. Biol. Sci., Northern Arizona Univ., Flagstaff 1998.
Murray, M.G., Thompson, W.F.: Rapid isolation of high molecular weight plant DNA.-Nucl. Acid. Res. 8: 4321–4325, 1980.
Narasimhan, S., Padmesh, P., Nair, G.M.: Assessment of genetic diversity in Coscinium fenestratum.-Biol. Plant. 50: 111–113, 2006.
Nei, M.: Estimation of average heterozygosity and genetic distance from a small number of individuals.-Genetics 89: 583–590, 1978.
Padmesh, P., Reji, J.V., Dhar, M.J., Seeni, S.: Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD.-Biol. Plant. 50: 367–372, 2006.
Wu, X., Liu, Q.H., Ma, Y.H. Dang, R.L.: [RAPD analysis of genetic relationship among different populations of Glycyrrhiza uralensis from six growing area in Sinkiang.]-Chin. J. Biochem. Pharm. 24(4): 191–193, 2003. [In Chin.]
Yeh, F.C., Young, R.C., Boyle, T.: Microsoft Window-based freeware for population genetic analysis (POPGENE, ver. 1.31). ftp://ftp.microsoft.com/softlib/mslfiles/hpgl.exe, 1999.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yao, H., Zhao, Y., Chen, D.F. et al. ISSR primer screening and preliminary evaluation of genetic diversity in wild populations of Gycyrrhiza uralensis . Biol Plant 52, 117–120 (2008). https://doi.org/10.1007/s10535-008-0022-7
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-008-0022-7