Abstract
A micropropagation protocol through multiple shoot formation was developed for Thlaspi caerulescens L., one of the most important heavy metals hyperaccumulator plants. In vitro seed-derived young seedlings were used for the initiation of multiple shoots on Murashige and Skoog (MS) medium with combinations of benzylaminopurine (BA; 0.5–1.0 mg dm−3), naphthaleneacetic acid (NAA; 0–0.2 mg dm−3), gibberellic acid (GA3; 0–1.0 mg dm−3) and riboflavin (0–3.0 mg dm−3). The maximum number of shoots was developed on medium containing 1.0 mg dm−3 BA and 0.2 mg dm−3 NAA. GA3 (0.5 mg dm−3) in combination with BA significantly increased shoot length. In view of shoot numbers, shoot length and further rooting rate, the best combination was 1.0 mg dm−3 BA + 0.5 mg dm−3 GA3 + 1.0 mg dm−3 riboflavin. Well-developed shoots (35–50 mm) were successfully rooted at approximately 95 % on MS medium containing 20 g dm−3 sucrose, 8 g dm−3 agar and 1.0 mg dm−3 indolebutyric acid. Almost all in vitro plantlets survived when transferred to pots.
Abbreviations
- BA:
-
benzylaminopurine
- GA3:
-
gibberellic acid
- IBA:
-
indolebutyric acid
- LH:
-
lactalbumin hydrolysate
- NAA:
-
naphthaleneacetic acid
- PGR:
-
plant growth regulators
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Xu, J., Zhang, Y.X., Chai, T.Y. et al. In vitro multiplication of heavy metals hyperaccumulator Thlaspi caerulescens . Biol Plant 52, 97–100 (2008). https://doi.org/10.1007/s10535-008-0016-5
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DOI: https://doi.org/10.1007/s10535-008-0016-5