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Callus production, somatic embryogenesis and plant regeneration of Lycium barbarum root explants

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Biologia Plantarum

Abstract

A new micropropagation system for Lycium barbarum (L.) was developed using root explants as starting material. Callus can be produced from root explants on Murashige and Skoog (MS) medium containing 0.2 mg dm−3 2,4-dichlorophenoxyacetic acid. After three subcultures on the same medium, callus was then transferred onto the MS medium supplemented with 500 mg dm−3 lactalbumin hydrolysate to induce somatic embryogenesis (SE). After 20 d, about 60 somatic embryos per 0.25 g(f.m.) of embryogenic callus were obtained but only about 10 % of embryos converted into plantlets. After acclimated in the greenhouse, all of the randomly selected plantlets had survived and were similar phenotypically to zygotic seedlings. In addition, the effects of irradiance, photoperiod, growth regulators, explant age and cold treatment on SE of root-derived callus were evaluated.

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Abbreviations

BA:

6-benzyladenine

CI medium:

the medium for callus induction

2,4-D:

2,4-dichlorophenoxyacetic acid

EI medium:

the medium for SE induction

GA3 :

gibberellic acid

MS:

Murashige and Skoog’s medium (1962)

NAA:

α-naphthalene-acetic acid

SE:

somatic embryogenesis

References

  • Akashi, R., Hoffmann-Tsay, S.S., Hoffmann, F.: Selection of a super-growing legume root culture that permits controlled switching between root cloning and direct embryogenesis.-Theor. appl. Genet. 96: 758–764, 1998.

    Article  CAS  Google Scholar 

  • Bekele, E., Klock, G., Zimmermann, U.: Somatic embryogenesis and plant regeneration from leaf and root explants and from seeds of Eragrostis tef (Gramineae).-Hereditas 123: 183–189, 1995

    Article  Google Scholar 

  • Bergmann, B.A., Kackett, W.P., Pellett, H.: Somatic embryogenesis in Aesculus.-In Vitro cell dev. Biol. Plant 32: 161–164, 1996.

    Article  Google Scholar 

  • Carman, J.G.: Embryogenic cell in plant tissue cultures: occurrence and behavior.-In Vitro cell dev. Biol. Plant 26: 746–753, 1990.

    Article  Google Scholar 

  • Chand, S., Sahrawat, A.K.: Somatic embryogenesis and plant regeneration from root segments of Psoralea corylifolia L., an endangered medicinally important plant.-In Vitro cell dev. Biol. Plant 38: 33–38, 2002.

    Article  Google Scholar 

  • Hess, J.R., Carman, J.G.: Embryogenic competence of immature wheat embryos: genotype, donor plant environment, and endogenous hormone levels.-Crop Sci. 38: 249–253, 1998.

    Article  CAS  Google Scholar 

  • Hu, Z., Ding, H.B., Wang, X., Wang, L.S.: [A comparative study on the syntheses of DNA, RNA and protein during in vitro organogenesis and somatic embryogenesis of Lycium barbarum.]-Acta Biol. exp. sin. 31: 403–411, 1998. [In Chinese.]

    CAS  Google Scholar 

  • Hu, Z., Guo, G.Q., Zhao, D.L., Li, L.H., Zheng, G.C.: Shoot regeneration from leaf explant of Lycium barbarum and Agrobalterium-mediated genetic transformation.-Russ. J. of Plant Physiol. 48: 453–458, 2001.

    Article  CAS  Google Scholar 

  • Hu, Z., Wang Y.X., Wu, Y. R., Li, W.: [Effects of light and plant growth regulators on growth of normal and hairy root from Lycium barbarum in vitro.] China J. Chinese Materia med. 31: 106–110, 2006. [In Chinese.]

    Google Scholar 

  • Hu, Z., Yang, J., Guo, G.Q., Zheng, G.C.: High efficiency transfomation of Lycium barbarum and Agrobacterium tumefaciens and transgenic plant regeneration via somatic embryogenesis.-Plant Cell Rep. 21: 233–237, 2002.

    Article  CAS  Google Scholar 

  • Jayasankar, S., Gray, D.J., Litz, R.E.: High-efficiency somatic embryogenesis and plant regeneration from suspension cultures of grapevine.-Plant Cell Rep. 18: 533–537, 1999.

    Article  CAS  Google Scholar 

  • Komai, F., Okuse, I., Harada, T.: Somatic embryogenesis and plant regeneration in culture of root segments of spinach (Spinacia oleracea L).-Plant Sci. 113: 203–208, 1996.

    Article  CAS  Google Scholar 

  • Luo, J.P., Jiang, S.T., Pan, L.J.: Cold-enhanced somatic embryogenesis in cell suspension cultures of Astragalus adsurgens Pall.: relationship with exogenous calcium during cold pretreatment.-Plant Growth Regul. 40: 171–177, 2003.

    Article  CAS  Google Scholar 

  • Milivojević, S., Mitrović, A., Ćulafić, Lj.: Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro.-Biol. Plant. 49: 35–39, 2005

    Article  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Niu, D.S., Shou, Q.Q., Qing, J.S., Wang, L., Wang, D.Z.: [Establishment and plant regeneration of single cell culture in Lycium barbarum.]-Chinese Sci. Bull. 30: 296–298, 1985. [In Chinese.]

    Google Scholar 

  • Niu, D.S., Shou, Q.Q., Wang, L., Qing, J.S., Cheng, S.P., Cao, S.F., Wang, D.Z.: [Plant regeneration from calli of hypocotyl, shoot and young ovary of Lycium barbarum.]-Hereditas (Beijing) 5: 24–26, 1983. [In Chinese.]

    Google Scholar 

  • Ratushnyak, Y.I., Piven, N.M., Rudas, V.A.: Protoplast culture and plant regeneration in Lycium barbarum L.-Plant Cell Tissue Organ Cult. 17: 183–190, 1989.

    Article  Google Scholar 

  • Ratushnyak, Y.I., Rudas, V.A., Piven, N.M.: Regeneration of Lycium barbarum L. plants from leaf tissue, callus culture and callus protoplast.-Plant Cell Rep. 9: 84–87, 1990.

    Article  Google Scholar 

  • Sharma, A., Kumar, A.: Somatic embryogenic and plant regeneration from leaf-derived cell suspension of a mature tree-Thevetia peruviana L.-Plant Cell Rep. 14: 171–174, 1994.

    Article  CAS  Google Scholar 

  • Wang, H.C., Chen, J.T., Wu, S.P., Lin, M.C., Chang, W.C.: Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu.-Biol. Plant. 50: 279–282, 2006.

    Article  Google Scholar 

  • Wang, H.Z., Du, L.Q., Huang, F.C., Li, A.S., Shou, Q.Q.: [Agrobacterium tumefaciens-mediated transformation of Lycium barbarum and regeneration of transgenic plants.]-Sci. China (Ser. B) 23: 391–395, 1993. [In Chinese.]

    Google Scholar 

  • Yu, M.S., Yuen, W.H., So, K.F., Chang, R.C.C.: Neuroprotective effects of extracts from anti-aging Chinese medicine Lycium barbarum (Gou-Qi-Zi).-J. Neurochem. 88(Suppl.): 69–69, 2004.

    Google Scholar 

  • Zhang, M., Chen, H.X., Huang, J., Li, Z., Zhu, C.P., Zhang, S.H.: Effect of Lycium barbarum polysaccharide on human hepatoma QGY7703 cells: Inhibition of proliferation and induction of apoptosis.-Life Sci. 76: 2115–2124, 2005.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Shantou University, Shantou, 515063, Guangdong Province, P.R. China

    Z. Hu, Y. Hu, X. Q. Guan & D. H. Zhuang

  2. Institute of Cell Biology, School of Life Science, Lanzhou University, Lanzhou, 730000, Gansu Province, P.R. China

    Z. Hu & H. H. Gao

Authors
  1. Z. Hu
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  2. Y. Hu
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  3. H. H. Gao
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  4. X. Q. Guan
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  5. D. H. Zhuang
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Correspondence to Z. Hu.

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Hu, Z., Hu, Y., Gao, H.H. et al. Callus production, somatic embryogenesis and plant regeneration of Lycium barbarum root explants. Biol Plant 52, 93–96 (2008). https://doi.org/10.1007/s10535-008-0015-6

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  • DOI: https://doi.org/10.1007/s10535-008-0015-6

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