Skip to main content
SpringerLink
Log in

Induction of alternative oxidase chain under salt stress conditions

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

This paper describes the effect of NaCl on the respiration of Citrus cell suspensions namely on the induction of the alternative oxidase. The exposure of two Citrus (cvs. Carvalhal tangor and Valencia late) cell suspensions to 200 or 400 mM NaCl lead to a reduction on cell respiration rates. Under these conditions, the respiration rate decreased less in the presence of KCN indicating a stimulation of the capacity of the alternative oxidase (AOX). In addition, immunoblots showed an increase on the amount of AOX protein. Antibodies raised against the Sauromatum guttatum enzyme recognized the reduced form of the enzyme near the 35 kDa band. The protein accumulation was correlated with the significantly higher AOX capacity observed for cv. Carvalhal tangor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AOX:

alternative oxidase

RD :

dark respiration rate

ROS:

reactive oxygen species

SHAM:

salicyl hydroxamic acid

References

  • Amthor, J.S.: The McCree-de Wit-Penning de Vries-Thornley respiration paradigms: 30 years later.-Ann. Bot. 86: 1–20, 2000.

    Article  CAS  Google Scholar 

  • Aubert, S., Gout, E., Bligny, R., Douce R.: Induction of alternative oxidase synthesis by herbicides inhibiting branched-chain amino acid synthesis.-Plant J. 11: 649–657, 1997.

    Article  CAS  Google Scholar 

  • Bartoli, C.G., Gomez, F., Gergoff, G., Guiamet, J.J., Puntarulo, S.: Up-regulation of the mitochondrial alternative oxidase enhances photosynthetic electron transport under drought conditions.-J. exp. Bot. 56: 1269–1276, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Duque P., Arrabaça J.D.: Respiratory metabolism during cold storage of apple fruit. II. Alternative oxidase is induced at the climacteric.-Physiol. Plant. 107: 24–31, 1999.

    Article  CAS  Google Scholar 

  • Elthon, T.E., Nickels, R.L., McIntosh, L.: Monoclonal antibodies to the alternative oxidase of higher plant mitochondria.-Plant Physiol. 89: 1311–1317, 1989.

    PubMed  CAS  Google Scholar 

  • Ferreira, A.L., Lima-Costa, M.E.: Metabolic responses to salt stress in cell suspension cultures of sensitive and resistant Citrus.-JHSB 81: 983–988, 2006.

    CAS  Google Scholar 

  • Fung, R.W.M., Wang, C.Y., Smith, D.L., Gross, K.C., Tian, M.: MeSA and MeJA increase steady-state transcript levels of alternative oxidase and resistance against chilling injury in sweet peppers (Capsicum annuum L.).-Plant Sci. 166: 711–719, 2004.

    Article  CAS  Google Scholar 

  • Geraldes-Laakso, S., Arrabaça, J.D.: Respiration and heat production by soybean hypocotyl and cotyledon mitochondria.-Plant Physiol. Biochem. 35: 897–903, 1997.

    CAS  Google Scholar 

  • González-Meler, M.A., Giles L, Thomas, R.B., Siedow, J.M.: Metabolic regulation of leaf respiration and alternative pathway activity in response to phosphate supply.-Plant Cell Environ. 24: 205–215, 2001

    Article  Google Scholar 

  • Hiser, C., McIntosh, L.: Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices.-Plant Physiol. 93: 312–318, 1990

    Article  PubMed  CAS  Google Scholar 

  • Jebara, M., Harzalli-Jebara, S., Payré, H., Aouani, M.E., Drevon, J.J.: Influence of salinity and abscisic acid on the O2 uptake by N2-fixing nodules of common bean.-Biol. Plant. 50: 717–721, 2006.

    Article  CAS  Google Scholar 

  • Kearns, A., Whelan, J., Young, S., Elthon, T.E., Day, D.A.: Tissue-specific expression of the alternative oxidase in soybean and siratro.-Plant Physiol. 99: 712–717, 1992.

    PubMed  CAS  Google Scholar 

  • Kirimura, K., Matsui, T., Sugano, S., Usami, S.: Enhancement and repression of cyanide-insensitive respiration in Aspergillus ringer.-FEMS Microbiol. Lett. 141: 251–254, 1996.

    Article  PubMed  CAS  Google Scholar 

  • Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680–686, 1970.

    Article  PubMed  CAS  Google Scholar 

  • Lennon, A.M., Neuenschwander, U.H., Ribas-Carbo, M., Giles, L., Ryals, J.A., Siedow, J.N.: The effects of salicylic acid and tobacco mosaic virus infection on the alternative oxidase of tobacco.-Plant Physiol. 115: 783–791, 1997.

    PubMed  CAS  Google Scholar 

  • Maxwell, D.P., Wang, Y., McIntosh, L.: The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells.-Proc. nat. Acad. Sci. USA 96: 8271–8276, 1999.

    Article  PubMed  CAS  Google Scholar 

  • Meeuse, B.J.D.: Thermogenic respiration in aroids.-Annu. Rev. Plant Physiol. 26: 117–126, 1975.

    Article  CAS  Google Scholar 

  • Millar, A.H., Wiskich, J.T., Whelan, J., Day, D.A.: Organic acid activation of the alternative oxidase of plant mitochondria.-FEBS Lett. 329: 259–262, 1993.

    Article  PubMed  CAS  Google Scholar 

  • Mittler, R.: Oxidative stress, antioxidants and stress tolerance.-Trends Plant Sci. 7: 405–410, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Mizuno, M., Tada, Y., Uchii, K., Kawakami, S., Mayama, S.: Catalase and alternative oxidase cooperatively regulate programmed cell death induced by β-glucan elicitor in potato suspension cultures.-Planta 220: 849–853, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Moore, A.L., Albury, M.S., Crichton, P.G., Affourtit, C.: Function of the alternative oxidase: is it a scanvenger?-Trends Plant Sci. 7: 478–481, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 12: 473–497, 1962.

    Article  Google Scholar 

  • Noguchi, K., Taylor, N.L., Millar, A.H., Lambers, H., Day, D.A.: Response of mitochondria to light intensity in the leaves of sun and shade species.-Plant Cell Environ. 28: 760–771, 2005.

    Article  CAS  Google Scholar 

  • Pádua, M., Aubert, S., Casimiro, A., Bligny, R., Millar, A.H., Day, D.A.: Induction of alternative oxidase by excess copper in sycamore cell suspensions.-Plant Physiol. Biochem. 37: 131–137, 1999.

    Article  Google Scholar 

  • Parani, M., Rudrabhatla, S., Myers, R., Weirich, H., Smith, B., Leaman, D.W., Goldman, S.L.: Microarray analysis of nitric oxide responsive transcripts in Arabidopsis.-Plant Biotechnol. J. 2: 359–366, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Purvis, A.C., Shewfelt, R.L.: Does the alternative pathway ameliorate chilling injury in sensitive plant tissues?-Physiol. Plant. 88: 712–718, 1993.

    Article  CAS  Google Scholar 

  • Rhoads, D.M., McIntosh, L.: Salicylic acid and regulation of respiration in higher plants, alternative oxidase expression.-Plant Cell 4: 1131–1139, 1992.

    Article  PubMed  CAS  Google Scholar 

  • Ribas-Carbo, M., Aroca, R., González-Meler, M.A., Irigoyen, J.J., Sanchez-Diaz, M.: The electron partitioning between the cytochrome and alternative respiratory pathways during chilling recovery in two cultivars of maize differing in chilling sensitivity.-Plant Physiol. 122: 199–204, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Rychter, A.M., Mikulska, M.: The relationship between phosphate status and cyanide-resistant respiration in bean roots.-Physiol. Plant. 79: 663–667, 1990.

    Article  CAS  Google Scholar 

  • Siedow, J.N., Umbach, A.L.: The mitochondrial cyanide-resistant oxidase: structural conservation amid regulatory diversity.-BBA-Bioenergetics 1459: 432–439, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Sieger, S.M., Kristensen, B.K., Robson, C.A., Amirsadeghi, S., Eng, E.W.Y., Abdel-Mesih, A., Møller, I.M., Vanlerberghe, G.C.: The role of alternative oxidase in modulating carbon use efficiency and growth during macronutrient stress in tobacco cells.-J. exp Bot. 56: 1499–1515, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Simons, B.H., Lambers, H.: The alternative oxidase: is it a respiratory pathway allowing a plant to cope with stress?-In: Lerner, H.R. (ed.): Plant Responses to Environmental Stresses: from Phytohormones to Genome Reorganization. Pp. 265–286. Marcel Dekker, New York 1999.

    Google Scholar 

  • Storey, R., Walker, R.R.: Citrus and salinity.-Sci. Hort. 78: 39–81, 1999.

    Article  CAS  Google Scholar 

  • Umbach, A.L., Wiskich, J.T., Siedow, J.N.: Regulation of alternative oxidase kinetics by puruvate and intermolecular disulfide bond redox status in soybean seedling mitochondria.-FEBS Lett. 348: 181–184, 1994.

    Article  PubMed  CAS  Google Scholar 

  • Vandenabeele, S., Van der Kelen, K., Dat, J., Gadjev, I., Boonefaes, T., Morsa, S., Rottiers, P., Slooten, L., Van Montagu, M., Zabeau, M., Inze, D., Van Breusegem, F.: A comprehensive analysis of hydrogen peroxideinduced gene expression in tobacco.-PNAS 100: 16113–16118, 2003.

    Article  PubMed  CAS  Google Scholar 

  • Vanlerberghe, G.C., McIntosh, L.: Lower growth temperature increases alternative pathway capacity and alternative oxidase protein in tobacco.-Plant Physiol. 100: 115–119, 1992a.

    PubMed  CAS  Google Scholar 

  • Vanlerberghe, G.C., McIntosh, L.: Coordinate regulation of cytochrome and alternative pathway respiration tobacco.-Plant Physiol. 100: 1846–1851, 1992b.

    PubMed  CAS  Google Scholar 

  • Vanlerberghe, G.C., McIntosh, L.: Mitochondrial electron transport regulation of nuclear gene expression. Studies with the alternative oxidase gene of tobacco.-Plant Physiol. 105: 867–874, 1994.

    Article  PubMed  CAS  Google Scholar 

  • Wagner, A.M.: A role of active oxygen species as second messengers in the induction of alternative oxidase gene expression in Petunia hybrida cells.-FEBS Lett. 368: 339–342, 1995.

    Article  PubMed  CAS  Google Scholar 

  • Wagner, A.M., Krab, K.: The alternative respiration pathway in plants: role and regulation.-Physiol. Plant. 95: 318–325, 1995.

    Article  CAS  Google Scholar 

  • Wagner, A.M., Moore, AL.: Structure and function of the plant alternative oxidase: its putative role in the oxygen defence mechanism.-Biosci. Rep. 17: 319–333, 1997.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculdade de Engenharia de Recursos Naturais, Universidade do Algarve, Campus de Gambelas, 8005-134, Gambelas, Portugal

    A. L. Ferreira & M. E. Lima-Costa

  2. Faculdade de Ciências, Universidade de Lisboa, Campo Grande Edifício C2, 1749-016, Lisboa, Portugal

    J. D. Arrabaça & V. Vaz-Pinto

Authors
  1. A. L. Ferreira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. D. Arrabaça
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Vaz-Pinto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. E. Lima-Costa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. L. Ferreira.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ferreira, A.L., Arrabaça, J.D., Vaz-Pinto, V. et al. Induction of alternative oxidase chain under salt stress conditions. Biol Plant 52, 66–71 (2008). https://doi.org/10.1007/s10535-008-0009-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-008-0009-4

Additional key words

Search

Navigation