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Improvement of protein quality in transgenic soybean plants

Biologia Plantarum volume 51, pages 277–284 (2007)Cite this article

Abstract

Glycinin is one of the abundant storage proteins in soybean seeds. A modified Gy1 (A1aB1b) proglycinin gene with a synthetic DNA encoding four continuous methionines (V3-1) was connected between the hpt gene and the modified green fluorescent protein sGFP(S65T) gene, and a resultant plasmid was introduced into soybean by particle bombardment in order to improve nutritional value of its seeds. After the selection with hygromycin, the efficiency of gene introduction was evaluated. More than 60 % of the regenerated plants tolerant to hygromycin yielded the hpt and V3-1 fragment by polymerase chain reaction (PCR) analysis, and the expression of sGFP was detected in about 50 % of putative transgenic soybeans. Southern hybridization confirmed the presence of transgenes in T0 plants and the transgenic soybeans hybridized with the hpt and V3-1 genes were analyzed showed different banding patterns. Most of the transgenic plants were growing, flowering normally and produced seeds. Analysis of seed obtained from transgenic soybean plants expressing hpt and V3-1 genes showed higher accumulation of glycinin compared with non-transgenic plants. In addition, protein expression in transgenic soybean plants was observed by using 2D-electrophoresis.

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Abbreviations

GFP:

green fluorescent protein

PCR:

polymerase chain reaction

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Authors and Affiliations

  1. Biochemistry Department, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt

    H. A. El-Shemy

  2. National Agricultural Research Center for Western Region, Fukuyama, Hiroshima, 721-8514, Japan

    H. A. El-Shemy, M. M. Khalafalla & M. Ishimoto

  3. Commission of Biotechnology and Genetic Engineering, National Center for Research, Khartoum, Sudan

    M. M. Khalafalla

  4. Graduate School of Biosphere Sciences, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan

    K. Fujita

Authors
  1. H. A. El-Shemy
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  2. M. M. Khalafalla
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  3. K. Fujita
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  4. M. Ishimoto
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Correspondence to H. A. El-Shemy.

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El-Shemy, H.A., Khalafalla, M.M., Fujita, K. et al. Improvement of protein quality in transgenic soybean plants. Biol Plant 51, 277–284 (2007). https://doi.org/10.1007/s10535-007-0055-3

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  • DOI: https://doi.org/10.1007/s10535-007-0055-3

Additional key words

  • genetic improvement
  • Glycine max
  • glycinin
  • particle bombardment
  • PCR
  • seed storage proteins