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Effects of sugars and growth regulators on in vitro growth of Dactylorhiza species

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Biologia Plantarum

Abstract

The influence of sugars and growth regulators on shoot and root growth of Dactylorhiza species was studied under in vitro conditions. The seedling development was stimulated with the application of glucose and sucrose at concentration of 10 g dm−3 each. The improvement of shoot growth rate and shoot length was enhanced by cytokinins N 6-(2-isopentenyl)adenine or N 6-benzyladenine and their combination with auxin indolebutyric acid (IBA). The root growth rate and root length of seedlings increased in the presence of IBA and α-naphthaleneacetic acid. Individual Dactylorhiza species showed statistically significant differences in shoot and root development depending on sugar and growth regulator combinations.

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Abbreviations

BA:

N 6-benzyladenine

IAA:

indole-3-acetic acid

IBA:

indolebutyric acid

2iP:

N 6-(2-isopentenyl)adenine

KIN:

kinetin (N 6-furfuryladenine)

NAA:

α-naphthaleneacetic acid

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Correspondence to K. Wotavová-Novotná.

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Wotavová-Novotná, K., Vejsadová, H. & Kindlmann, P. Effects of sugars and growth regulators on in vitro growth of Dactylorhiza species. Biol Plant 51, 198–200 (2007). https://doi.org/10.1007/s10535-007-0040-x

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  • DOI: https://doi.org/10.1007/s10535-007-0040-x

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