Abstract
Mauritanian date palm cultivars and progenies of two controlled crosses were analyzed according to the identity of mitochondrial plasmid-like DNAs. Starting from total genomic DNA and appropriate primers, polymerase chain reaction was designed to amplify either a 373-bp or a 265-bp fragments corresponding to the S and the R-plasmid respectively. Data proved that 5 cultivars out of 10 studied have exhibited the R-plasmid suggesting their resistance to the fusariosis. The existence of intra-cultivar variability has also been revealed in the cv. Ahmar. In addition, analysis throughout progenies of two controlled crosses suggested the strict maternal transmission of the date palms’ mitochondrial genome.
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Abbreviations
- BSA:
-
bovine serum albumin
- PCR:
-
polymerase chain reaction
- PEG:
-
polyethylene glycol
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Ould Mohamed Salem, A., Rhouma, S., Zehdi, S. et al. Molecular characterization of Mauritanian date palm cultivars using plasmid-like DNAs markers. Biol Plant 51, 169–172 (2007). https://doi.org/10.1007/s10535-007-0033-9
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DOI: https://doi.org/10.1007/s10535-007-0033-9