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Effect of drought on biomass, protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars

Abstract

The effects of drought on growth, protein content, lipid peroxidation, superoxide dismutase (SOD), peroxidase (POX), catalase (CAT) and polyphenol oxidase (PPO) were studied in leaves and roots of Sesamum indicum L. cvs. Darab 14 and Yekta. Four weeks after sowing, plants were grown under soil moisture corresponding to 100, 75, 50 and 25 % field capacity for next four weeks. Fresh and dry masses, and total protein content in leaves and roots decreased obviously under drought. However, several new proteins appeared and content of some proteins was affected. Measurement of malondialdehyde content in leaves and roots showed that lipid peroxidation was lower in Yekta than in Darab 14. Severe stress increased SOD, POX, CAT and PPO activities in leaves and roots, especially in Yekta. According to the present study Yekta is more resistant to drought than Darab 14.

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Abbreviations

CAT:

catalase

FC:

field capacity

L-DOPA:

L-3,4-dihydroxyphenylalanine

MDA:

malondialdehyde

NBT:

nitroblue tetrazolium

POX:

peroxidase

PPO:

polyphenol oxidase

ROS:

reactive oxygen species

SDS-PAGE:

sodium dodecyl-sulphate polyacrylamide gel electrophoresis

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

TCA:

trichloroacetic acid

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Correspondence to F. Fazeli.

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Fazeli, F., Ghorbanli, M. & Niknam, V. Effect of drought on biomass, protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars. Biol Plant 51, 98–103 (2007). https://doi.org/10.1007/s10535-007-0020-1

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Additional key words

  • catalase
  • malondialdehyde
  • peroxidase
  • polyphenol oxidase
  • Sesamum indicum
  • superoxide dismutase