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Superoxide dismutase activity in C3 and C3/CAM intermediate species of Clusia

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Biologia Plantarum

Abstract

The C3-CAM intermediate Clusia minor L. and the C3 obligate Clusia multiflora H.B.K. plants were exposed for 7 d to a combination of drought stress and high irradiance of about 1200 µmol m−2 s−1 for 12 h per day. In both species under these conditions a strong decrease in stomatal conductance was observed at dawn and dusk. Changes in stomatal behaviour of C. minor were accompanied by only a low nocturnal accumulation of malate and citrate. Thus, in C. minor drought stress applied in combination with high irradiance limited CAM expression, and possibly this is the main reason why C. minor prefers semi-shaded sites in the field. The mitochondrial MnSOD, in both well watered and stressed plants of two species showed strong diurnal oscillations with maximum activity at dusk. These oscillations can be explained by the engagement of mitochondria in dissipation of an excess of reducing equivalents. In plants which are able to carry out CAM metabolism tricarboxylic acid cycle is expected to be down regulated in the dark period to prevent breakdown of the entire malate and citrate.

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Abbreviations

A:

antheraxanthin

BSA:

bovine serum albumin

COX:

cytochrome c oxidase

CuZnSOD:

CuZn-superoxide dismutase

DTT:

dithiothreitol

EPS:

epoxidation state of the xanthophyll cycle pigments

gs :

stomatal conductance

H2EDTA:

ethylenediaminetetraacetic acid

HEPES:

N-(2-hydroxyethyl)piperazine-N′-(ethanesulphonic acid)

MnSOD:

Mn-superoxide dismutase

PAGE:

polyacrylamide gel electrophoresis

PEG:

polyethylene glycol

PEPC:

phosphoenolpyruvate carboxylase

PFD:

photon flux density

PVPP:

polyvinylpolypyrrolidone

ROS:

reactive oxygen species

TCA cycle:

tricarboxylic acid cycle

V:

violaxanthin

Z:

zeaxanthin

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Authors and Affiliations

  1. Institute of Biology, Pedagogical University, ul. Podbrzezie 3, PL-31054, Krakow, Poland

    Z. Miszalski, A. Kornas & K. Gawronska

  2. Institute of Plant Physiology, Polish Academy of Sciences, PL-30239, Krakow, Poland

    Z. Miszalski, I. Ślesak & E. Niewiadomska

  3. Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, PL-30387, Krakow, Poland

    J. Kruk

  4. Institute of Botany, Darmstadt University of Technology, D-64287, Darmstadt, Germany

    A. L. Christian, E. Fischer-Schliebs, R. Krisch & U. Lüttge

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  1. Z. Miszalski
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  2. A. Kornas
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  3. K. Gawronska
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  4. I. Ślesak
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  5. E. Niewiadomska
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  10. U. Lüttge
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Correspondence to Z. Miszalski.

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Miszalski, Z., Kornas, A., Gawronska, K. et al. Superoxide dismutase activity in C3 and C3/CAM intermediate species of Clusia . Biol Plant 51, 86–92 (2007). https://doi.org/10.1007/s10535-007-0018-8

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  • DOI: https://doi.org/10.1007/s10535-007-0018-8

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