Abstract
Following drought stress at supraoptimal temperature the increase in proline (Pro) content in transgenic (T) soybean [Glycine max (L.) Merr. cv. Ibis] plants overexpressing the gene coding for the last enzyme of Pro biosynthesis, L-Δ1-pyrroline-5-carboxylate reductase, was much greater than in wild type (W) plants (105-fold versus 19-fold after 7 d). Under control conditions arginine accounted for nearly 60 % of the total free amino acid content. After stress treatment the content of Pro was more than 50 % in both T and W genotypes, and at the end of recovery the γ-aminobutyrate content reached 27 and 53 % in the W and T plants, respectively. Without stress treatment there was only a 2-fold difference between T and W in the tyrosine content. However, during the stress period and the subsequent recovery a similar difference was found for many amino acids. The present results indicate that manipulating of the content of a single amino acid influences the whole free amino acid composition in soybean.
Abbreviations
- Arg:
-
arginine
- Asn:
-
asparagine
- Asp:
-
aspartate
- DM:
-
dry mass
- Gaba:
-
γ-aminobutyrate
- Gln:
-
glutamine
- Glu:
-
glutamate
- His:
-
histidine
- Ile:
-
isoleucine
- Leu:
-
leucine
- Phe:
-
phenylalanine
- P5CR:
-
L-Δ1-pyrroline-5-carboxylate reductase
- Pro:
-
proline
- PS:
-
preliminary stress
- RWC:
-
relative water content
- Ser:
-
serine
- T:
-
transgenic plants
- Tyr:
-
tyrosine
- W:
-
wild type
References
Bandurska, H.: Does Pro accumulated in leaves of water deficit stressed barley plants confine cell membrane injury? I. Free Pro accumulation and membrane injury index in drought and osmotically stressed plants.-Acta Physiol. Plant. 22: 409–415, 2000.
Behl, R.K., Moawad, A.M., Achtmich, W.: Amino acid and protein profile changes in spring wheat mutant under prolonged heat stress.-Ann. Biol. Ludhiana 7: 63–67, 1991.
Chandra, A., Pathak, P.S., Bhatt, R.K., Dubey, A.: Variation in drought tolerance of different Stylosanthes accessions.-Biol. Plant. 48: 457–460, 2004.
De Ronde, J.A., Laurie, R.N., Caetano, T., Greyling, M.M., Kerepesi, I.: Comparative study between transgenic and non-transgenic soybean lines proved transgenic lines to be more drought tolerant.-Euphytica 138: 123–132, 2004.
De Ronde, J.A., Spreeth, M.H., Cress, W.A.: Effect of antisense L-Δ1-pyrroline-5-carboxylate reductase transgenic soybean plants subjected to osmotic and drought stress.-Plant Growth Regul. 32: 13–26, 2000.
El Tayeb, M.A., Hassanein, A.M.: Germination, seedling growth, some organic solutes and peroxidase expression of different Vicia faba lines as influenced by water stress.-Acta agron. hung. 48: 11–20, 2000.
Galiba, G., Kocsy, G., Kaur-Sawhney, R., Sutka, J., Galston, A.W.: Chromosomal localisation of osmotic and salt stress-induced differential alterations in polyamine content in wheat.-Plant Sci. 92: 203–211, 1993.
Galiba, G., Simon-Sarkadi, L., Salgó, A., Kocsy, G.: Genotype dependent adaptation of wheat varieties to water stress in vitro.-J. Plant Physiol. 134: 730–735, 1989.
Good, A.G., Zaplachinski, S.T.: The effects of drought stress on free amino acid accumulation and protein synthesis in Brassica napus.-Physiol. Plant. 90: 9–14, 1994.
Nayyar, H., Walia, D.P.: Water stress-induced Pro accumulation in contrasting wheat genotypes as affected by calcium and abscisic acid.-Biol. Plant. 46: 275–279, 2003.
Raggi, V.: Changes in free amino acids and osmotic adjustment in leaves of water-stressed bean.-Physiol. Plant. 91: 427–434, 1994.
Trotel-Aziz, P., Niogret, M.F., Deleu, C., Bouchereau, A., Aziz, A., Larher, F.R.: The control of Pro consumption by abscisic acid during osmotic stress recovery of canola leaf discs.-Physiol. Plant. 117: 213–221, 2003.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Simon-Sarkadi, L., Kocsy, G., Várhegyi, Á. et al. Stress-induced changes in the free amino acid composition in transgenic soybean plants having increased proline content. Biol Plant 50, 793–796 (2006). https://doi.org/10.1007/s10535-006-0134-x
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-006-0134-x