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Protein profile in the transgenic kiwifruit overexpressing a transcription factor gene, OSH1


The aim of this study was to investigate the protein alterations in the transgenic kiwifruit (Actinidia deliciosa) overexpressing a transcription factor gene, OSH1. Although transgenic plant with introduced OSH1 indicates suppression of gibberellin (GA)-20 oxidase activity, application of GA to transgenic kiwifruit could not completely recover plant morphology and protein profiles. Eleven proteins decreased in the transgenic kiwifruit detected by two-dimensional polyacrylamide gel electrophoresis showed homologies to kiwifruit hypothetical protein, osmotin I and photosynthesis related protein. These results suggest that introduction of an OSH1 into kiwifruit caused wide-range alterations at protein level and alterations of protein accumulation should be considered to evaluate the substantial equivalence of plants transformed by a transcription factor.

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Coomassie brilliant blue


two-dimensional polyacrylamide gel electrophoresis




isoelectric focusing


polyvinylidene difluoride


sodium dodecyl sulfate polyacrylamide gel electrophoresis


  • Celis, J.E., Bravo R. (ed): Two-Dimensional Gel Electrophoresis of Proteins Methods and Applications.-Academic Press, New York 1984.

    Google Scholar 

  • Cleveland, D.W., Fischer, S.G., Kirschner, M.W., Laemmeli, U.K.: Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.-J. biol. Chem. 252: 1102–1106, 1977.

    PubMed  CAS  Google Scholar 

  • Damerval, C., Vienne, D., Zivy, M., Thiellement, H.: Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins.-Electrophoresis 7: 52–54, 1986.

    CAS  Article  Google Scholar 

  • Dell’Aquila, A.: Protein patterns, characterized by computer image analysis, of lentil embryo axes germinating under salt stress.-Biol. Plant. 48: 237–242, 2004.

    CAS  Article  Google Scholar 

  • Gehring, W.J.: Homeoboxes in the study of development.-Science 236: 1245–1252, 1987.

    PubMed  CAS  Google Scholar 

  • Gygi, S.P., Corthals, G.L., Zhang, Y., Rochon, Y., Aebersold, R.: Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology.-Proc. nat. Acad. Sci. USA 97: 9390–9395, 2000.

    PubMed  CAS  Article  Google Scholar 

  • Kano-Murakami, Y., Yanai, T., Tagiri, A. Matsuoka, M.: A rice homeotic gene, OSH1, causes unusual phenotypes in transgenic tobacco.-FEBS Lett. 334: 365–368, 1993.

    PubMed  CAS  Article  Google Scholar 

  • Komatsu, S., Yang, G., Unno, K., Park, P.: Characterization of a membrane-associated phosphoprotein (pp47) in rice (Oryza sativa L.) seedlings treated by gibberellin.-J. Plant Physiol. 159: 121–128, 2002.

    CAS  Article  Google Scholar 

  • Konishi, H., Ishiguro, K., Komatsu, S.: A proteomics approach towards understanding blast fungus infection of rice grown under different levels of nitrogen fertilization.-Proteomics 1: 1162–1171, 2001.

    PubMed  CAS  Article  Google Scholar 

  • Kusaba, S., Fukumoto, M., Honda, C., Yamaguchi, I., Sakamoto, T. Kano-Murakami, Y.: Decreased GA1 content caused by the overexpression of OSH1 is accompanied by suppression of GA20-oxidase gene expression.-Plant Physiol. 117: 1179–1184, 1998.

    PubMed  CAS  Article  Google Scholar 

  • Kusaba, S., Kano-Murakami, Y., Matsuoka, M., Matsuta, N., Sakamoto, T., Fukumoto, M.: Expression of the rice homeobox gene, OSH1, causes morphological changes in transgenic kiwifruit.-J. jap. Soc. hort. Sci. 68: 482–486, 1999.

    CAS  Article  Google Scholar 

  • Ledger, S.E., Gardner, R.C.: Cloning and characterization of five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia deliciosa var. deliciosa).-Plant mol. Biol. 25: 877–886, 1994.

    PubMed  CAS  Article  Google Scholar 

  • Marenholz, I., Zirra, M., Fischer, D.F., Backendorf, C., Ziegler, A., Mischke, D.: Identification of human epidermal differentiation complex (EDC)-encoded genes by subtractive hybridization of entire YACs to a gridded keratinocyte cDNA library.-Genome Res. 11: 341–355, 2001.

    PubMed  CAS  Article  Google Scholar 

  • Matsuoka, M., Tamaoki, M., Tada, Y., Fuyjimura, T., Tagiri, A., Yamamoto, N., Kano-Murakami, Y.: Expression of rice OSH1 gene is localized in developing vascular strands and its ectopic expression in transgenic rice causes altered morphology of leaf.-Plant Cell Rep. 14: 555–559, 1995.

    CAS  Article  Google Scholar 

  • O’Farrell, P.H.: High resolution two-dimensional electrophoresis of proteins.-J. biol. Chem. 250: 4007–4021, 1975.

    PubMed  CAS  Google Scholar 

  • Sakamoto, T., Kamiya, N., Ueguchi-Tanaka, M., Iwahori, S., Matsuoka, M.: KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem.-Gene Dev. 15: 581–590, 2001.

    PubMed  CAS  Article  Google Scholar 

  • Scott, M.P., Tamkun, J.W., Hartzell, G.W., III.: The structure and function of the homeodomain.-Biochem. biophys. Acta 989: 25–48, 1989.

    PubMed  CAS  Google Scholar 

  • Singh, N.K., Bracker, C.A., Hasegawa, P.M., Handa, A.K., Buckel, S., Hermodson, M.A., Pfankoch, E., Regnier, F.E., Bressan, R.A.: Characterization of osmotin, a thaumatin-like protein associated with osmotic adaptation in plant cells.-Plant Physiol. 85: 529–536, 1987.

    PubMed  CAS  Article  Google Scholar 

  • Tamaoki, M., Yamamoto, N., Matsuoka, M.: Isolation and characterization of cDNA clones with enhanced expression in tobacco plants expressing the rice homeobox gene, OSH1.-Plant Cell Physiol. 38: 638–642, 1997.

    PubMed  CAS  Google Scholar 

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Correspondence to M. Kita.

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Kita, M., Honda, C., Komatsu, S. et al. Protein profile in the transgenic kiwifruit overexpressing a transcription factor gene, OSH1 . Biol Plant 50, 759–762 (2006).

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Additional key words

  • Actinidia deliciosa
  • 2D-PAGE
  • gibberellin oxidase
  • gibberellins
  • proteomics