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Protein profile in the transgenic kiwifruit overexpressing a transcription factor gene, OSH1

Abstract

The aim of this study was to investigate the protein alterations in the transgenic kiwifruit (Actinidia deliciosa) overexpressing a transcription factor gene, OSH1. Although transgenic plant with introduced OSH1 indicates suppression of gibberellin (GA)-20 oxidase activity, application of GA to transgenic kiwifruit could not completely recover plant morphology and protein profiles. Eleven proteins decreased in the transgenic kiwifruit detected by two-dimensional polyacrylamide gel electrophoresis showed homologies to kiwifruit hypothetical protein, osmotin I and photosynthesis related protein. These results suggest that introduction of an OSH1 into kiwifruit caused wide-range alterations at protein level and alterations of protein accumulation should be considered to evaluate the substantial equivalence of plants transformed by a transcription factor.

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Abbreviations

CBB:

Coomassie brilliant blue

2D-PAGE:

two-dimensional polyacrylamide gel electrophoresis

GA:

gibberellin

IEF:

isoelectric focusing

PVDF:

polyvinylidene difluoride

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Correspondence to M. Kita.

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Kita, M., Honda, C., Komatsu, S. et al. Protein profile in the transgenic kiwifruit overexpressing a transcription factor gene, OSH1 . Biol Plant 50, 759–762 (2006). https://doi.org/10.1007/s10535-006-0125-y

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  • DOI: https://doi.org/10.1007/s10535-006-0125-y

Additional key words

  • Actinidia deliciosa
  • 2D-PAGE
  • gibberellin oxidase
  • gibberellins
  • proteomics