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Polyamine contents, ethylene synthesis, and BrACO2 expression during turnip germination

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Biologia Plantarum

Abstract

Contents of total free [PA(S)] and conjugated polyamines [PA(SH), PA(PH)] were higher in turnip (Brassica rapa L. cv. Rapa) seeds during imbibition (0–36 h) and radicle protrusion (36–48 h) than during the further growth (10 d). Ethylene production was activated with the protrusion, reaching a maximum at the second day of germination and dropping afterwards. The application of ethrel accelerated radicle emergence but the direct intervention of ethylene in the breaking of the seed coat was not clear from the use of ethylene-biosynthesis inhibitors (CoCl2 and AVG). Finally, in this work the gene BrACO2 was characterized. Although its expression was not detected in seeds through zygotic embryogenesis, it increased concomitantly with the germination process.

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Abbreviations

ACO:

1-aminocyclopropane-1-carboxylate oxidase

AdoMet:

S-adenosyl-L-methionine

AVG:

L-α-(2-aminoethoxyvinyl) glycine

Cad:

cadaverine

Ethrel:

2-chloroethylphosphonic acid

PA:

polyamine

PA(PH):

acid insoluble conjugated polyamine

Put:

putrescine

PA(S):

free-polyamine

PA(SH):

acid soluble conjugated polyamine

Spd:

spermidine

Spm:

spermine

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Authors and Affiliations

  1. Lab. Fisiología Vegetal, Facultade de Biología, Edificio de Ciencias Experimentales, Universidad de Vigo, E-36310, Vigo, Spain

    M. I. Puga-Hermida & M. Gallardo

  2. Lab. Fisiología Vegetal, Escuela Politécnica Superior, Universidad de Santiago de Compostela, E-15782, Santiago de Compostela, Spain

    M. C. Rodriguez-Gacio

  3. Lab. Fisiología Vegetal, Facultade de Farmacia, Universidad de Santiago de Compostela, E-15782, Santiago de Compostela, Spain

    A. J. Matilla

Authors
  1. M. I. Puga-Hermida
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  2. M. Gallardo
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  3. M. C. Rodriguez-Gacio
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  4. A. J. Matilla
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Correspondence to A. J. Matilla.

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Puga-Hermida, M.I., Gallardo, M., Rodriguez-Gacio, M.C. et al. Polyamine contents, ethylene synthesis, and BrACO2 expression during turnip germination. Biol Plant 50, 574–580 (2006). https://doi.org/10.1007/s10535-006-0090-5

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  • DOI: https://doi.org/10.1007/s10535-006-0090-5

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