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Effects of ion channel inhibitors on cold- and electrically-induced action potentials in Dionaea muscipula

Biologia Plantarum volume 50, pages 411–416 (2006)Cite this article

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Abstract

Glass microelectrodes were inserted into Dionaea muscipula (Venus flytrap) lobes and the action potentials (APs) were recorded in response to a sudden temperature drop or a direct current (DC) application. The effect of potassium channel inhibitor, tetraethylammonium ion, was the lengthening of the depolarization phase of AP. APs were also affected by the anion channel inhibitor, anthracene-9-carboxylic acid, that made them slower and smaller. Neomycin, which disturbs inositol triphosphate-dependent Ca2+ release, caused the visible inhibition of AP, too. Ruthenium red, which blocks cyclic ADP-ribose-dependent Ca2+ release, totally inhibited DC-triggered APs and induced the decrease in the amplitudes of cold-evoked APs. Lanthanum ions significantly inhibited both cold- and DC-induced membrane potential changes. It was concluded that during excitation Dionaea muscipula relied upon the calcium influxes from both the extra- and intracellular compartments.

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Abbreviations

A:

amplitude

AP:

action potential

A-9-C:

anthracene-9-carboxylic acid

cADPR:

cyclic ADP-ribose

CICR:

calcium induced calcium release

IP3 :

inositol triphosphate

PLC:

phospholipase C

RP:

resting potential

RyR:

ryanodine receptor

t1/2 :

half-time of action potential

TEA+ :

tetraethylammonium ion

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Authors and Affiliations

  1. Department of Biophysics, Institute of Biology, Maria Curie-Sklodowska University, Akademicka 19, PL-20033, Lublin, Poland

    E. Krol, H. Dziubinska, M. Stolarz & K. Trebacz

Authors
  1. E. Krol
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  2. H. Dziubinska
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  3. M. Stolarz
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  4. K. Trebacz
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Correspondence to E. Krol.

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Krol, E., Dziubinska, H., Stolarz, M. et al. Effects of ion channel inhibitors on cold- and electrically-induced action potentials in Dionaea muscipula . Biol Plant 50, 411–416 (2006). https://doi.org/10.1007/s10535-006-0058-5

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  • DOI: https://doi.org/10.1007/s10535-006-0058-5

Additional key words

  • calcium
  • cyclic ADP-ribose
  • inositol triphosphate
  • phospholipase C
  • tetraethylammonium ion
  • Venus flytrap