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Peroxidase activity and lignification in soybean root growth-inhibition by juglone

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Biologia Plantarum

Abstract

The changes in activities of soluble and cell wall-bound peroxidases and lignin contents in juglone-stressed soybean (Glycine max) seedlings and their relationships with root growth were investigated. Soybean seedlings (3-d-old) were cultivated in nutrient solution supplemented with 0.5 to 25 μM juglone for 24 h. Length and dry mass of roots decreased after 5 to 25 μM juglone treatments. Low juglone concentrations (≤ 1 μM) increased soluble peroxidase activity, while high concentrations (≥ 10 μM) inhibited activities of soluble and cell wall-bound peroxidases. Juglone (≤ 1 μM) did not affect lignin content but highly increased lignification after 5 to 25 μM treatments. Results indicate that lignification may be an important step in root growth reduction of juglone-stressed soybean.

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Abbreviations

d.m.:

dry mass

ROS:

reactive oxygen species

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Authors and Affiliations

  1. Department of Biochemistry, University of Maringa, Av. Colombo, 5790, 87020-900, Maringa, PR, Brazil

    P. A. F. Bohm, F. M. L. Zanardo, M. L. L. Ferrarese & O. Ferrarese-Filho

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  1. P. A. F. Bohm
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  2. F. M. L. Zanardo
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  3. M. L. L. Ferrarese
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  4. O. Ferrarese-Filho
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Correspondence to O. Ferrarese-Filho.

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Bohm, P.A.F., Zanardo, F.M.L., Ferrarese, M.L.L. et al. Peroxidase activity and lignification in soybean root growth-inhibition by juglone. Biol Plant 50, 315–317 (2006). https://doi.org/10.1007/s10535-006-0029-x

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  • DOI: https://doi.org/10.1007/s10535-006-0029-x

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