Abstract
Plant regeneration through somatic embryogenesis of Areca catechu L. was established using leaf, root and stem segments as explants. Embryogenic callus was induced and maintained on medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 3,6-dichloro-2-methoxybenzoic acid (dicamba) at concentrations 2, 4, 6 and 8 mg dm−3 in darkness. Somatic embryos were found on primary callus in the presence of 2 and 4 mg dm−3 dicamba and during subculture on 2 – 8 mg dm−3 2,4-D or 2 – 4 mg dm−3 dicamba-containing media. Plantlet conversion from embryos was successfully achieved on growth regulator-free medium. The plants grew well when transplanted to containers in shaded greenhouse.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- dicamba:
-
3,6-dichloro-2-methoxybenzoic acid
- MS:
-
Murashige and Skoog (1962) medium
- BA:
-
N6-benzyladenine
- TDZ:
-
1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron)
References
Bhaskaran, S., Smith, R.H.: Somatic embryogenesis from shoot tip and immature infloresence of Phoenix dactylifera cv. Barhee.-Plant Cell Rep. 12: 22–25, 1992.
Chen, J.T., Chang, W.C.: Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis.-Biol. Plant. 50: in press, 2006.
Dias, A.C., Guerra, M.P., Cordoba, A.S., Kemper, E.L.: Somatic embryogenesis and plant regeneration in the tissue culture of Genoma gamiova (Arecaceae).-Acta Hort. 360: 167–171, 1994.
Fernando, S.C., Gamage, C.K.A.: Abscisic acid induced somatic embryogenesis in immature embryo explants of coconut (Cocos nucifera L.).-Plant Sci. 151: 193–198, 2000.
Gabr, M.F., Tisserat, B.: Propagating palms in vitro with special emphasis on the date palm (Phoenix dactylifera).-Sci. Hort. 25: 255–262, 1985.
Ganapathi, T.R., Suprasanna, P., Bapat, V., Rao P.S.: In vitro culture of embryos of areca nut (Areca catechu L.).-Fruits 52: 313–316, 1997.
Guerra, M.P., Handro, W.: Somatic embryogenesis and plant regeneration in different organs of Euterpe edulis Mart. (Palmae): Control and structural features.-J. Plant Res. 111: 65–71, 1998.
Jensen, W.A.: Botanical Histochemistry.-Freeman, San Francisco 1962.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant 15: 495–497, 1962.
Su, Y.J., Chen, J.T., Chang, W.C.: Efficient and repetitive production of leaf-derived somatic embryos of Oncidium.-Biol. Plant. 50: in press, 2006.
Wang, H.C., Chen, J.T., Wu, S.P., Lin, M.C., Chang, W.C.: Plant regeneration through somatic embryogenesis from zygotic embryo-derived callus of Areca cathecu L. (Arecaceae).-In Vitro cell. dev. Biol. Plant 39: 34–36, 2002.
Wang, H.C., Chen, J.T., Wu, S.P., Lin, M.C., Chang, W.C.: Plant regeneration through shoot formation from callus of Areca cathecu L.-Plant Cell Tissue Organ Cult. 75: 95–98, 2003.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, H.C., Chen, J.T. & Chang, W.C. Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu . Biol Plant 50, 279–282 (2006). https://doi.org/10.1007/s10535-006-0020-6
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-006-0020-6