Biologia Plantarum

, Volume 50, Issue 2, pp 227–231 | Cite as

Antioxidant defense mechanism under salt stress in wheat seedlings

  • S. Mandhania
  • S. MadanEmail author
  • V. Sawhney


The present study was carried out to study the effect of salt stress on cell membrane damage, ion content and antioxidant enzymes in wheat (Triticum aestivum) seedlings of two cultivars salt-tolerant KRL-19 and salt-sensitive WH-542. Seedlings (4-d-old) were irrigated with 0, 50 and 100 mM NaCl. Observations were recorded on the 3rd and 6th day after salt treatment and 2nd day after salt removal. The relative water content declined with induction of salt stress, more in WH-542 than in cv. KRL-19. K+/Na+ ratio in KRL-19 was higher than in WH-542. WH-542 suffered greater damage to cellular membranes due to lipid peroxidation as indicated by higher accumulation of H2O2, MDA and greater leakage of electrolytes than KRL-19. The activities of catalase, peroxidase and ascorbate peroxidase and glutathione reductase increased with increase in salt stress in both the cultivars, however, superoxide dismutase activity declined. Upon desalanization, partial recovery in the activities of these enzymes was observed in KRL-19 and very slow recovery in WH-542.

Additional key words

ascorbate peroxidase calatase glutathione reductase hydrogen peroxide malondialdehyde peroxidase superoxide dismutase Triticum aestivum 



ascorbate peroxidase




days after removal of salt


electrical conductivity


days after salt treatment


ethylenediaminetetraacetic acid


glutathione reductase


oxidised glutathione




nicotinamide adenine dinucleotide phosphate (reduced)


nitroblue tetrazolium




relative water content


superoxide dismutase


trichloroacetic acid


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Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2006

Authors and Affiliations

  1. 1.Department of Plant BreedingCCS Haryana Agricultural UniversityHisarIndia

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