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Biologia Plantarum

, Volume 50, Issue 2, pp 227–231 | Cite as

Antioxidant defense mechanism under salt stress in wheat seedlings

  • S. Mandhania
  • S. Madan
  • V. Sawhney
Article

Abstract

The present study was carried out to study the effect of salt stress on cell membrane damage, ion content and antioxidant enzymes in wheat (Triticum aestivum) seedlings of two cultivars salt-tolerant KRL-19 and salt-sensitive WH-542. Seedlings (4-d-old) were irrigated with 0, 50 and 100 mM NaCl. Observations were recorded on the 3rd and 6th day after salt treatment and 2nd day after salt removal. The relative water content declined with induction of salt stress, more in WH-542 than in cv. KRL-19. K+/Na+ ratio in KRL-19 was higher than in WH-542. WH-542 suffered greater damage to cellular membranes due to lipid peroxidation as indicated by higher accumulation of H2O2, MDA and greater leakage of electrolytes than KRL-19. The activities of catalase, peroxidase and ascorbate peroxidase and glutathione reductase increased with increase in salt stress in both the cultivars, however, superoxide dismutase activity declined. Upon desalanization, partial recovery in the activities of these enzymes was observed in KRL-19 and very slow recovery in WH-542.

Additional key words

ascorbate peroxidase calatase glutathione reductase hydrogen peroxide malondialdehyde peroxidase superoxide dismutase Triticum aestivum 

Abbreviations

APX

ascorbate peroxidase

CAT

catalase

DAR

days after removal of salt

EC

electrical conductivity

DAT

days after salt treatment

EDTA

ethylenediaminetetraacetic acid

GR

glutathione reductase

GSSG

oxidised glutathione

MDA

malondialdehyde

NADPH

nicotinamide adenine dinucleotide phosphate (reduced)

NBT

nitroblue tetrazolium

POD

peroxidase

RWC

relative water content

SOD

superoxide dismutase

TCA

trichloroacetic acid

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References

  1. Alscher, R.G., Donahue, J.C., Cramer, C.L.: Reactive oxygen species and antioxidants: relationship in green cells.-Physiol. Plant. 100: 224–233, 1997.CrossRefGoogle Scholar
  2. Benavides, M.P., Marconi, P.L., Gallego, S.M., Comba, M.E., Tomaro, M.L.: Relationship between antioxidant defense systems and salt tolerance in Solanum tuberosum.-Aust. J. Plant. Physiol. 27: 273–278, 2000.Google Scholar
  3. Cheeseman, J.M.: Mechanism of salinity tolerance in plants.-Plant Physiol. 87: 547–550, 1988.CrossRefPubMedGoogle Scholar
  4. Dalmia, A., Sawhney, V.: Antioxidant defense mechanism under drought stress in wheat seedlings.-Physiol. mol. Biol. Plants 10: 109–114, 2004.Google Scholar
  5. Dionisio-Sese, M.L., Tobita, S.: Antioxidant response of rice seedlings to salinity stress.-Plant Sci. 135: 1–9, 1998.CrossRefGoogle Scholar
  6. Dureja, V.: Effect of salinity stress on antioxidant enzymes in salt-tolerant and salt-sensitive cultivar of rice (Oryza sativa L.).-M.Sc. Thesis. CCS Haryana Agricultural Univerisity, Hisar 2003.Google Scholar
  7. Foyer, C.H., Lopez-Delgado, H., Dat, J.F., Scott, I.M.: Hydrogen peroxide and glutathione-associated mechanisms of acclimatory stress tolerance and signalling.-Physiol. Plant. 100: 241–254, 1997.CrossRefGoogle Scholar
  8. Giannopolitis, C.N., Ries, S.K.: Superoxide dismutases II: Purification and quantitative relationship with water soluble protein in seedlings.-Plant Physiol. 59: 315–318, 1977.PubMedGoogle Scholar
  9. Gossett, D.R., Milhollon, E.P., Lucas, M.C.: Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivar of cotton.-Crop Sci. 34: 706–714, 1994.CrossRefGoogle Scholar
  10. Gueta-Dahan, Y., Yaniv, Z., Zilinskas, B.A., Ben-Hayyim, G.: Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in citrus.-Planta 203: 460–469, 1997.PubMedCrossRefGoogle Scholar
  11. Halliwel, B., Foyer, C.H.: Properties and physiological functions of a glutathione reductase purified from spinach leaves by affinity chromatography.-Planta 139: 9–17, 1978.Google Scholar
  12. Heath, R.L., Packer, I.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.-Arch. Biochem. Biophys. 125: 189–198, 1968.PubMedGoogle Scholar
  13. Jackson, M.L.: Soil Chemical Analysis.-Prentice Hall of India Pvt. Ltd., New Delhi 1973.Google Scholar
  14. Kukreja, S.: Physiological studies on chickpea (Cicer arietinum L.) genotypes under saline conditions.-Ph.D. Thesis. CCS Haryana Agricultural University, Hisar 2003.Google Scholar
  15. Lutts, S., Majerus, V., Kinet, J.M.: NaCl effects on proline metabolism in rice (Oryza sativa) seedlings.-Physiol. Plant. 105: 450–458, 1999.CrossRefGoogle Scholar
  16. Molina, A., Bueno, P., Marin, M.C., Rodriguez-Rosales, M.P., Belver, A., Venema, K., Donaire, J.P.: Involvement of endogenous salicylic acid content, lipoxygenase and antioxidant enzyme activities in the response of tomato cell suspension culture to NaCl.-New Phytol. 156: 409–415, 2002.CrossRefGoogle Scholar
  17. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867–880, 1981.Google Scholar
  18. Noctor, G., Foyer, C.H.: Ascorbate and glutathione: keeping active oxygen under control.-Annu. Rev. Plant Physiol. Plant mol. Biol. 49: 249–279, 1998.PubMedCrossRefGoogle Scholar
  19. Panda, S.K., Upadhyay, R.K.: Salt stress injury induces oxidative alteration and antioxidative defence in the roots of Lemna minor.-Biol. Plant. 48: 249–253, 2003.Google Scholar
  20. Pandey, R., Ganapathy, P.S.: Effect of sodium chloride stress on callus culture of Cicer arientinum L. cv. BG-203. Growth and ion accumulation.-J. exp. Bot. 35: 1194–1199, 1984.Google Scholar
  21. Racagni, H., Pedranzani, H., Alemano, S., Taleisnik, E., Abdala, G., Machado-Domenech, E.: Effect of short-term salinity on lipid metabolism and ion accumulation in tomato roots.-Biol. Plant. 47: 373–377, 2003/4.Google Scholar
  22. Rout, M.P., Shaw, B.P.: Salt tolerance in aquatic macrophytes: possible involvement of the antioxidative enzymes.-Plant Sci. 160: 415–423, 2001.PubMedCrossRefGoogle Scholar
  23. Sairam, R.K., Rao, K.V., Srivastava, G.C.: Differential response of wheat genotypes to long-term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration.-Plant Sci. 163: 1037–1046, 2002.CrossRefGoogle Scholar
  24. Sairam, R.K., Srivastava, G.C., Agarwal, S., Meena, R.C.: Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes.-Biol. Plant. 49: 85–91, 2005.CrossRefGoogle Scholar
  25. Shannon, L.M., Key, E., Law, J.Y.: Peroxidase isozymes from horse reddish roots: isolation and physical properties.-J. biol. Chem. 241: 2166–2172, 1966.PubMedGoogle Scholar
  26. Sinha, A.K.: Colorimetric assay of catalase.-Anal. Biochem. 47: 389–395: 1972.PubMedCrossRefGoogle Scholar
  27. Sreenivasulu, N., Grimm, B., Wobus, U., Weschke, W.: Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of foxtail millet (Setaria italica).-Physiol. Plant. 109: 435–442, 2000.CrossRefGoogle Scholar
  28. Vranova, E., Inze, D., Van Breusegem, F.: Signal transduction during oxidative stress.-J. exp. Bot. 53: 1227–1236, 2002.PubMedCrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2006

Authors and Affiliations

  1. 1.Department of Plant BreedingCCS Haryana Agricultural UniversityHisarIndia

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