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Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings

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Biologia Plantarum

Abstract

Protein pattern, ammonia content, glutamine synthetase activity, lipid peroxidation, superoxide dismutase, catalase, peroxidase and peroxidase isoforms were studied in the leaves and roots of 7-d-old peanut (Arachis hypogaea L. cv. JL-24) seedlings treated by 25, 100 and 250 μM jasmonic acid (JA). SDS-PAGE protein profile of leaves and roots after JA application showed a significant increase in 18, 21, 30, 45, 47 and 97.4 kDa proteins and significant decrease in 22 and 36 kDa proteins. Pathogenesis related PR-18 was specific in leaves at 250 μM JA and PR-21 have cross reacted differently with 21 and 30 kDa proteins in leaves and roots treated by all JA concentrations. Further, the immunoblot analysis with glutamine synthetase, GS-45 antibodies revealed a specific cross reaction with 45 and 47 kDa proteins of both control and JA treated leaves, however, higher at 100 and 250 μM JA treated leaves than control ones. Further, the malondialdehyde (MDA) content significantly increased in leaves and roots treated with JA, indicated membrane damage with JA treatments that led to the generation of peroxidation products. The peroxidase isozymic pattern showed two specific isoforms. Besides, the activities of SOD and catalase were significantly elevated in JA treated leaves.

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Abbreviations

CAT:

catalase

GDH:

glutamate dehydrogenase

GOGAT:

glutamate synthase

GS:

glutamine synthetase

JA:

jasmonic acid

MDA:

malondialdehyde

MeJA:

methyl jasmonate

POD:

peroxidase

PR:

pathogenesis related

PVDF:

polyvinylidene difluoride

ROS:

reactive oxygen species

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Department of Botany and Biotechnology, Sri Krishnadevaraya University, Anatapur, 515003, India

    G. J. Kumari, S. T. Naik, J. Prasanthi, G. Sriranganayakulu, P. C. Reddy & Chinta Sudhakar

  2. Institute of Plant Genetics and Crop Plant Research, Gatersleben, D-06466, Germany

    S. G. Kumar

  3. College of Pharmacy, Chungbuk National University, Cheongju, 361-763, South Korea

    A. M. Reddy

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  1. G. J. Kumari
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  4. S. G. Kumar
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  6. G. Sriranganayakulu
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  8. Chinta Sudhakar
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Correspondence to Chinta Sudhakar.

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Kumari, G.J., Reddy, A.M., Naik, S.T. et al. Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings. Biol Plant 50, 219–226 (2006). https://doi.org/10.1007/s10535-006-0010-8

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  • DOI: https://doi.org/10.1007/s10535-006-0010-8

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