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Effects of different N-sources on growth, nutritional status, chlorophyll content, and photosynthetic parameters of shoots of the apple rootstock MM 106 cultured in vitro

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Biologia Plantarum

Abstract

The effects of five different N-sources (KNO3 + NH4NO3 = control, KNO3, NH4NO3, NH4H2PO4, L-alanine) on growth, nutritional status, chlorophyll (Chl) content, and photosynthetic parameters of the apple rootstock MM 106 shoots cultured in vitro were investigated. In comparison to all the other treatments, control explants grown on a MS medium containing KNO3 + NH4NO3 had the highest fresh mass, Chl content, net photosynthetic rate, transpiration rate, and stomatal conductance.

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Abbreviations

Chl:

chlorophyll

MS:

Murashige and Skoog

Rubisco:

ribulose-1,5-bisphosphate carboxylase/oxygenase

References

  • Avilla, A., Pereira, S.M., Arguello, J.A.: Nitrogen concentration and proportion of NH4 +-N affect potato cultivar response in solid and liquid media.-HortScience 33: 336–338, 1998.

    Google Scholar 

  • Chen, J.J., Liao, Y.J.: Nitrogen-induced changes in the growth and metabolism of cultured potato tubers.-J. amer. Soc. hort. Sci. 118: 831–834, 1993.

    Google Scholar 

  • Filner, P.: Regulation of nitrate reductase in cultured tobacco cells.-Biochem. biophys. Acta. 118: 299–310, 1966.

    PubMed  CAS  Google Scholar 

  • Guidi, L., Lorefice, G., Pardossi, A., Malorgio, F., Tognoni, F., Soldatini, G.F.: Growth and photosynthesis of Lycopersicon esculentum (L.) plants as affected by nitrogen deficiency.-Biol. Plant. 40: 235–244, 1998.

    Google Scholar 

  • Hidder, C., Vezina, L.P., Desjardins, Y.: Short-term studies of 15NO3 − and 15NH4 + uptake by micropropagated strawberry shoots cultured with or without CO2 enrichment.-Plant Cell Tissue Organ Cult. 37: 185–191, 1994.

    Google Scholar 

  • Hsu, S.Y., Hsu, Y.T., Kao, C.H.: Ammonium ion, ethylene, and abscisic acid in polyethylene glycol-treated rice leaves.-Biol. Plant. 46: 239–242, 2003.

    CAS  Google Scholar 

  • Jain, V., Pal, M., Lakkineni, K.C., Abrol, Y.P.: Photosynthetic characteristics in two wheat genotypes as affected by nitrogen nutrition.-Biol. Plant. 42: 217–222, 1999.

    CAS  Google Scholar 

  • Mashayekhi-Nezamabadi, K.: The Protein Synthesis Spectrum During the Induction Phase of Somatic Embryogenesis in Carrot (Daucus carota L.) Cultures and the Role of Nitrogen Forms for Embryo Development.-Dr. Sci. Thesis. Justus Liebig University, Giessen 2000.

    Google Scholar 

  • Mengel, K.: Ernährung und Stoffwechsel der Pflanze.-Gustav Fisher Verlag, Jena 1991.

    Google Scholar 

  • Mordhorst, A.P., Lorz, H.: Embryogenesis and development of isolated barley (Hordeum vulgare L.) microspores are influenced by the amount and composition of nitrogen sources in culture media.-Plant Physiol. 142: 485–492, 1993.

    CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Plant Physiol. 15: 473–497, 1962.

    CAS  Google Scholar 

  • Neumann, K..: Pflanzliche Zell und Gewebekulturen.-Ulmer, Stuttgart 1995.

    Google Scholar 

  • Niedz, R.P.: Growth of embryogenic sweet orange callus on media varying in the ratio of nitrate to ammonium nitrogen.-Plant Cell Tissue Organ Cult. 39: 1–5, 1994.

    CAS  Google Scholar 

  • Olsen, F.L.: Induction of microspore embryogenesis in cultured anthers of Hordeum vulgare L. The effects of ammonium nitrate, glutamine and asparagine as nitrogen sources.-Carlsberg Res. Commun. 52: 393–404, 1987.

    Article  CAS  Google Scholar 

  • Preece, J.E.: Can nutrient salts partially substitute for plant growth regulators?-Plant Tissue Cult. Biotechnol. 1: 26–37, 1995.

    Google Scholar 

  • Stuart, D.A., Strickland, S.G.: Somatic embryogenesis from cell cultures of Medicago sativa L. I. The role of amino acid additions to the regeneration medium.-Plant Sci. Lett. 34: 165–174, 1984.

    CAS  Google Scholar 

  • Wolf, B.: Improvement in the azomethine-H method for the determination of boron.-Commun. Soil Sci. Plant Anal. 5: 39–44, 1974.

    Article  CAS  Google Scholar 

  • Yan, F., Schubert, S., Mengel, K.: Effect of low root medium pH on net proton release, root respiration and root growth of corn and broad bean.-Plant Physiol. 99: 415–421, 1992.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. N.AG.RE.F., Pomology Institute, P.O. Box 122, GR-59200, Naoussa, Greece

    T. E. Sotiropoulos, G. N. Mouhtaridou & T. Thomidis

  2. Laboratory of Pomology, School of Agriculture, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece

    V. Tsirakoglou, K. N. Dimassi & I. N. Therios

Authors
  1. T. E. Sotiropoulos
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  2. G. N. Mouhtaridou
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  3. T. Thomidis
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  4. V. Tsirakoglou
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  5. K. N. Dimassi
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  6. I. N. Therios
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Correspondence to T. E. Sotiropoulos.

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Sotiropoulos, T.E., Mouhtaridou, G.N., Thomidis, T. et al. Effects of different N-sources on growth, nutritional status, chlorophyll content, and photosynthetic parameters of shoots of the apple rootstock MM 106 cultured in vitro . Biol Plant 49, 297–299 (2005). https://doi.org/10.1007/s10535-005-7299-6

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  • DOI: https://doi.org/10.1007/s10535-005-7299-6

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