Abstract
Experiments were performed to optimize the macronutrients concentrations for in vitro rooting of Ceratonia siliqua micropropagated shoots. Several dilutions of Murashige and Skoog (MS) medium were tested: full-strength MS, half-strength MS (\({1 \mathord{\left/ {\vphantom {1 2}} \right. \kern-\nulldelimiterspace} 2}\)MS), and \({1 \mathord{\left/ {\vphantom {1 2}} \right. \kern-\nulldelimiterspace} 2}\)MS + full N. The frequency of in vitro rooting was enhanced when the \({1 \mathord{\left/ {\vphantom {1 2}} \right. \kern-\nulldelimiterspace} 2}\)MS was used (50 % rooted shoots). Mature leaves from 20 − 30 year-old carob trees and from 2 year-old micropropagated plants were collected and the concentrations of macronutrients (N, P, K, Ca, Mg) assessed. Based on the mineral composition of the leaves a new medium was formulated and compared with the previous ones showing an increment of the rooting frequency to 80 %. Moreover, shoots rooted in the new medium did not show symptoms of apical necrosis that occurred in the other tested media.
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Abbreviations
- BA:
-
benzyladenine
- IBA:
-
indole-3-butyric acid
- MS medium:
-
Murashige and Skoog (1962) medium
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Gonçalves, S., Correia, P.J., Martins-Loução, M.A. et al. A new medium formulation for in vitro rooting of carob tree based on leaf macronutrients concentrations. Biol Plant 49, 277–280 (2005). https://doi.org/10.1007/s10535-005-7280-4
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DOI: https://doi.org/10.1007/s10535-005-7280-4